Genetics, Vol 133, 499-508, Copyright © 1993

INVESTIGATIONS

A Ubiquitin-Conjugating Enzyme, RAD6, Affects the Distribution of Ty1 Retrotransposon Integration Positions

S. W. Liebman and G. Newnam
Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Box 4348, Chicago, Illinois 60680

A galactose-inducible Ty1 element was used to generate 59 independent Ty1 inserts that inactivate the CAN1 gene. As found in previous studies, the distribution of these elements shows a gradient of insertion frequency from highest to lowest between the 5' and 3' ends of the gene. However, 53 independent Ty1 and Ty2 insertions isolated by an identical procedure in an isogenic rad6 deletion strain do not show this bias. In this strain, the Ty elements insert randomly throughout CAN1. These results show that the ubiquitin-conjugating enzyme, RAD6, alters the integration site preferences of Ty1 retrotransposons.

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