Efficient and Dispersed Local P Element Transposition From Drosophila Females

INVESTIGATIONS

Efficient and Dispersed Local P Element Transposition From Drosophila Females

P. Zhang and A. C. Spradling
Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Baltimore, Maryland 21210

We have investigated how Drosophila P element insertions are distributed in the chromosomal region near their starting site. A single P element residing in the euchromatin of minichromosome Dp1187 was mobilized following a cross to the {Delta}2-3 (99B) strain, and progeny bearing transpositions were identified with a minimum of bias by performing Southern blots on progeny. Approximately 1-2% of all progeny minichromosomes contained new insertions. Many of these ``local transpositions'' landed very close to or within the starting P element; however, nearly 1% of all progeny chromosomes contained new insertions 1-180 kb from the donor element. More local insertions were observed in the progeny of females than from male parents, and most occurred in a preferred orientation relative to the starting element. These observations suggested that donor elements are frequently excised and reinserted locally without ever dissociating from a transposition complex. The high frequency and diverse distribution of local transpositions recovered from females suggested that the efficiency of insertional mutagenesis can be significantly enhanced by using a starting P element(s) located near the target of interest.

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				H. Eggert, K. Bergemann, and H. Saumweber Molecular Screening for P-Element Insertions in a Large Genomic Region of Drosophila melanogaster Using Polymerase Chain Reaction Mediated by the Vectorette Genetics, July 1, 1998; 149(3): 1427 - 1434. [Abstract] [Full Text] [PDF]

				J. G. Gindhart Jr., C. J. Desai, S. Beushausen, K. Zinn, and L. S.B. Goldstein Kinesin Light Chains Are Essential for Axonal Transport in Drosophila J. Cell Biol., April 20, 1998; 141(2): 443 - 454. [Abstract] [Full Text] [PDF]

				C. Dye, J. Lee, R. Atkinson, R Brewster, P. Han, and H. Bellen The Drosophila sanpodo gene controls sibling cell fate and encodes a tropomodulin homolog, an actin/tropomyosin-associated protein Development, January 5, 1998; 125(10): 1845 - 1856. [Abstract] [PDF]

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				S.-F. Tsai, C.-C. Jang, G. G. Prikhod'ko, D. A. Bessarab, C.-Y. Tang, G. O. Pflugfelder, and Y. H. Sun Gypsy retrotransposon as a tool for the in vivo analysis of the regulatory region of the optomotor-blind gene in Drosophila PNAS, April 15, 1997; 94(8): 3837 - 3831. [Abstract] [Full Text] [PDF]

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				Y Guo, A Gillan, T Torok, I Kiss, J A Dow, and K Kaiser Site-selected mutagenesis of the Drosophila second chromosome via plasmid rescue of lethal P-element insertions. Genome Res., October 1, 1996; 6(10): 972 - 979. [Abstract] [PDF]

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