- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Holbeck, S. L.
- Articles by Smith, G. R.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Holbeck, S. L.
- Articles by Smith, G. R.
Genetics, Vol 132, 879-891, Copyright © 1992
INVESTIGATIONS |
Chi Enhances Heteroduplex DNA Levels During Recombination
S. L. Holbeck and G. R. Smith
Present address: NCI-Frederick Cancer Research Facility, P.O. Box B, Building 539-152E, Frederick, Maryland 21701.
The major pathway of homologous recombination in Escherichia coli, the RecBCD pathway, is stimulated by Chi sites. To determine whether Chi enhances an early or late step in recombination, we measured formation of heteroduplex DNA (hDNA) in extracts of lambda-infected E. coli. Chi elevated hDNA levels in these extracts, supporting a role for Chi early (before hDNA formation) in recombination. RecA protein and RecBCD enzyme were both necessary for detection of hDNA, indicating that they, too, act early. Analysis of a panel of recBCD mutants indicated that Chi-nicking activity was needed for Chi's stimulation of hDNA formation. These results support a previously proposed model of recombination. Further results suggested that RecBCD enzyme has an additional role late in recombination.
This article has been cited by other articles:
 |
|
 |
|
  J. A. Nickoloff, D. B. Sweetser, J. A. Clikeman, G. J. Khalsa, and S. L. Wheeler Multiple Heterologies Increase Mitotic Double-Strand Break-Induced Allelic Gene Conversion Tract Lengths in Yeast Genetics, October 1, 1999; 153(2): 665 - 679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Evidence for Independent Mismatch Repair Processing on Opposite Sides of a Double-Strand Break in Saccharomyces cerevisiae Genetics, January 1, 1998; 148(1): 59 - 70.
|
|
|
|
 |
|
 A. F. Taylor and G. R. Smith Strand Specificity of Nicking of DNA at Chi Sites by RecBCD Enzyme J. Biol. Chem., October 13, 1995; 270(41): 24459 - 24467. [Abstract] [Full Text] [PDF]
|
|