Characterization of Suppressor of fused, a Complete Suppressor of the fused Segment Polarity Gene of Drosophila melanogaster

INVESTIGATIONS

Characterization of Suppressor of fused, a Complete Suppressor of the fused Segment Polarity Gene of Drosophila melanogaster

T. Preat
Present address: Theodor-Boveri-Institut fur Biossenschaften, der Universitat Wurzburg, Lehrsthuhl fur Genetik, D-8700 Wurzburg, Germany.

fused (fu) is a maternal effect segment polarity gene of Drosophila melanogaster. In addition, fu females have tumorous ovaries. Two ethyl methanesulfonate mutageneses were carried out in order to isolate suppressors of the fu phenotype. A new gene, Suppressor of fused (Su(fu)), was identified. It is located in the 87C8 region of the third chromosome. Su(fu) displays a maternal effect and is also expressed later in development. Although Su(fu)(LP) is a complete loss-of-function mutation, it is homozygous viable and has no phenotype by itself. Su(fu) fully suppresses the embryonic and adult phenotypes of fu mutants. Su(fu) mutations are semidominant and a Su(fu)(+) duplication has an opposite effect, enhancing the fused phenotype. It is proposed therefore that the Su(fu)(+) product is involved in the same developmental step as the Fu(+) kinase. Thus, a new gene interacting with the segment polarity pathway was identified using an indirect approach.

This article has been cited by other articles:

G. R. van den Brink
Hedgehog Signaling in Development and Homeostasis of the Gastrointestinal Tract
Physiol Rev, October 1, 2007; 87(4): 1343 - 1375.
[Abstract] [Full Text] [PDF]

Y. Liu, X. Cao, J. Jiang, and J. Jia
Fused Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation
Genes & Dev., August 1, 2007; 21(15): 1949 - 1963.
[Abstract] [Full Text] [PDF]

S. Martin-Lanneree, C. Lasbleiz, M. Sanial, S. Fouix, F. Besse, H. Tricoire, and A. Plessis
Characterization of the Drosophila myeloid leukemia factor
Genes Cells, December 1, 2006; 11(12): 1317 - 1335.
[Abstract] [Full Text] [PDF]

D. Huangfu and K. V. Anderson
Signaling from Smo to Ci/Gli: conservation and divergence of Hedgehog pathways from Drosophila to vertebrates
Development, January 1, 2006; 133(1): 3 - 14.
[Abstract] [Full Text] [PDF]

A. F. Cooper, K. P. Yu, M. Brueckner, L. L. Brailey, L. Johnson, J. M. McGrath, and A. E. Bale
Cardiac and CNS defects in a mouse with targeted disruption of suppressor of fused
Development, October 1, 2005; 132(19): 4407 - 4417.
[Abstract] [Full Text] [PDF]

M.-H. Chen, N. Gao, T. Kawakami, and P.-T. Chuang
Mice Deficient in the Fused Homolog Do Not Exhibit Phenotypes Indicative of Perturbed Hedgehog Signaling during Embryonic Development
Mol. Cell. Biol., August 15, 2005; 25(16): 7042 - 7053.
[Abstract] [Full Text] [PDF]

M. Merchant, M. Evangelista, S.-M. Luoh, G. D. Frantz, S. Chalasani, R. A. D. Carano, M. van Hoy, J. Ramirez, A. K. Ogasawara, L. M. McFarland, et al.
Loss of the Serine/Threonine Kinase Fused Results in Postnatal Growth Defects and Lethality Due to Progressive Hydrocephalus
Mol. Cell. Biol., August 15, 2005; 25(16): 7054 - 7068.
[Abstract] [Full Text] [PDF]

C. Zhang, E. H. Williams, Y. Guo, L. Lum, and P. A. Beachy
Inaugural Article: Extensive phosphorylation of Smoothened in Hedgehog pathway activation
PNAS, December 28, 2004; 101(52): 17900 - 17907.
[Abstract] [Full Text] [PDF]

M. Ascano Jr. and D. J. Robbins
An Intramolecular Association between Two Domains of the Protein Kinase Fused Is Necessary for Hedgehog Signaling
Mol. Cell. Biol., December 1, 2004; 24(23): 10397 - 10405.
[Abstract] [Full Text] [PDF]

L. Lum and P. A. Beachy
The Hedgehog Response Network: Sensors, Switches, and Routers
Science, June 18, 2004; 304(5678): 1755 - 1759.
[Abstract] [Full Text] [PDF]

J. Jia, C. Tong, and J. Jiang
Smoothened transduces Hedgehog signal by physically interacting with Costal2/Fused complex through its C-terminal tail
Genes & Dev., November 1, 2003; 17(21): 2709 - 2720.
[Abstract] [Full Text] [PDF]

L. Lum, S. Yao, B. Mozer, A. Rovescalli, D. Von Kessler, M. Nirenberg, and P. A. Beachy
Identification of Hedgehog Pathway Components by RNAi in Drosophila Cultured Cells
Science, March 28, 2003; 299(5615): 2039 - 2045.
[Abstract] [Full Text] [PDF]

F. Besse, D. Busson, and A.-M. Pret
Fused-dependent Hedgehog signal transduction is required for somatic cell differentiation during Drosophila egg chamber formation
Development, September 1, 2002; 129(17): 4111 - 4124.
[Abstract] [Full Text] [PDF]

K. E. Nybakken, C. W. Turck, D. J. Robbins, and J. M. Bishop
Hedgehog-stimulated Phosphorylation of the Kinesin-related Protein Costal2 Is Mediated by the Serine/Threonine Kinase Fused
J. Biol. Chem., June 28, 2002; 277(27): 24638 - 24647.
[Abstract] [Full Text] [PDF]

S. Y. Cheng and J. M. Bishop
Suppressor of Fused represses Gli-mediated transcription by recruiting the SAP18-mSin3 corepressor complex
PNAS, April 16, 2002; 99(8): 5442 - 5447.
[Abstract] [Full Text] [PDF]

M. Ascano Jr., K. E. Nybakken, J. Sosinski, M. A. Stegman, and D. J. Robbins
The Carboxyl-Terminal Domain of the Protein Kinase Fused Can Function as a Dominant Inhibitor of Hedgehog Signaling
Mol. Cell. Biol., March 1, 2002; 22(5): 1555 - 1566.
[Abstract] [Full Text] [PDF]

G. Wang, K. Amanai, B. Wang, and J. Jiang
Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus
Genes & Dev., November 15, 2000; 14(22): 2893 - 2905.
[Abstract] [Full Text]

N Methot and K Basler
Suppressor of fused opposes hedgehog signal transduction by impeding nuclear accumulation of the activator form of Cubitus interruptus
Development, January 9, 2000; 127(18): 4001 - 4010.
[Abstract] [PDF]

G. Wang, B. Wang, and J. Jiang
Protein kinase A antagonizes Hedgehog signaling by regulating both the activator and repressor forms of Cubitus interruptus
Genes & Dev., November 1, 1999; 13(21): 2828 - 2837.
[Abstract] [Full Text]

D. Stone, M Murone, S Luoh, W Ye, M. Armanini, A Gurney, H Phillips, J Brush, A Goddard, F. de Sauvage, et al.
Characterization of the human suppressor of fused, a negative regulator of the zinc-finger transcription factor Gli
J. Cell Sci., January 12, 1999; 112(23): 4437 - 4448.
[Abstract] [PDF]

J. T. Ohlmeyer and D. Kalderon
Dual pathways for induction of wingless expression by protein kinase A and Hedgehog in Drosophila embryos
Genes & Dev., September 1, 1997; 11(17): 2250 - 2258.
[Abstract] [Full Text] [PDF]

M. A. Stegman, J. E. Vallance, G. Elangovan, J. Sosinski, Y. Cheng, and D. J. Robbins
Identification of a Tetrameric Hedgehog Signaling Complex
J. Biol. Chem., July 14, 2000; 275(29): 21809 - 21812.
[Abstract] [Full Text] [PDF]

				Y. Liu, X. Cao, J. Jiang, and J. Jia Fused Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation Genes & Dev., August 1, 2007; 21(15): 1949 - 1963. [Abstract] [Full Text] [PDF]

				S. Martin-Lanneree, C. Lasbleiz, M. Sanial, S. Fouix, F. Besse, H. Tricoire, and A. Plessis Characterization of the Drosophila myeloid leukemia factor Genes Cells, December 1, 2006; 11(12): 1317 - 1335. [Abstract] [Full Text] [PDF]

				D. Huangfu and K. V. Anderson Signaling from Smo to Ci/Gli: conservation and divergence of Hedgehog pathways from Drosophila to vertebrates Development, January 1, 2006; 133(1): 3 - 14. [Abstract] [Full Text] [PDF]

				A. F. Cooper, K. P. Yu, M. Brueckner, L. L. Brailey, L. Johnson, J. M. McGrath, and A. E. Bale Cardiac and CNS defects in a mouse with targeted disruption of suppressor of fused Development, October 1, 2005; 132(19): 4407 - 4417. [Abstract] [Full Text] [PDF]

				M.-H. Chen, N. Gao, T. Kawakami, and P.-T. Chuang Mice Deficient in the Fused Homolog Do Not Exhibit Phenotypes Indicative of Perturbed Hedgehog Signaling during Embryonic Development Mol. Cell. Biol., August 15, 2005; 25(16): 7042 - 7053. [Abstract] [Full Text] [PDF]

				M. Merchant, M. Evangelista, S.-M. Luoh, G. D. Frantz, S. Chalasani, R. A. D. Carano, M. van Hoy, J. Ramirez, A. K. Ogasawara, L. M. McFarland, et al. Loss of the Serine/Threonine Kinase Fused Results in Postnatal Growth Defects and Lethality Due to Progressive Hydrocephalus Mol. Cell. Biol., August 15, 2005; 25(16): 7054 - 7068. [Abstract] [Full Text] [PDF]

				C. Zhang, E. H. Williams, Y. Guo, L. Lum, and P. A. Beachy Inaugural Article: Extensive phosphorylation of Smoothened in Hedgehog pathway activation PNAS, December 28, 2004; 101(52): 17900 - 17907. [Abstract] [Full Text] [PDF]

				M. Ascano Jr. and D. J. Robbins An Intramolecular Association between Two Domains of the Protein Kinase Fused Is Necessary for Hedgehog Signaling Mol. Cell. Biol., December 1, 2004; 24(23): 10397 - 10405. [Abstract] [Full Text] [PDF]

				L. Lum and P. A. Beachy The Hedgehog Response Network: Sensors, Switches, and Routers Science, June 18, 2004; 304(5678): 1755 - 1759. [Abstract] [Full Text] [PDF]

				J. Jia, C. Tong, and J. Jiang Smoothened transduces Hedgehog signal by physically interacting with Costal2/Fused complex through its C-terminal tail Genes & Dev., November 1, 2003; 17(21): 2709 - 2720. [Abstract] [Full Text] [PDF]

				L. Lum, S. Yao, B. Mozer, A. Rovescalli, D. Von Kessler, M. Nirenberg, and P. A. Beachy Identification of Hedgehog Pathway Components by RNAi in Drosophila Cultured Cells Science, March 28, 2003; 299(5615): 2039 - 2045. [Abstract] [Full Text] [PDF]

				F. Besse, D. Busson, and A.-M. Pret Fused-dependent Hedgehog signal transduction is required for somatic cell differentiation during Drosophila egg chamber formation Development, September 1, 2002; 129(17): 4111 - 4124. [Abstract] [Full Text] [PDF]

				K. E. Nybakken, C. W. Turck, D. J. Robbins, and J. M. Bishop Hedgehog-stimulated Phosphorylation of the Kinesin-related Protein Costal2 Is Mediated by the Serine/Threonine Kinase Fused J. Biol. Chem., June 28, 2002; 277(27): 24638 - 24647. [Abstract] [Full Text] [PDF]

				S. Y. Cheng and J. M. Bishop Suppressor of Fused represses Gli-mediated transcription by recruiting the SAP18-mSin3 corepressor complex PNAS, April 16, 2002; 99(8): 5442 - 5447. [Abstract] [Full Text] [PDF]

				M. Ascano Jr., K. E. Nybakken, J. Sosinski, M. A. Stegman, and D. J. Robbins The Carboxyl-Terminal Domain of the Protein Kinase Fused Can Function as a Dominant Inhibitor of Hedgehog Signaling Mol. Cell. Biol., March 1, 2002; 22(5): 1555 - 1566. [Abstract] [Full Text] [PDF]

				G. Wang, K. Amanai, B. Wang, and J. Jiang Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus Genes & Dev., November 15, 2000; 14(22): 2893 - 2905. [Abstract] [Full Text]

				N Methot and K Basler Suppressor of fused opposes hedgehog signal transduction by impeding nuclear accumulation of the activator form of Cubitus interruptus Development, January 9, 2000; 127(18): 4001 - 4010. [Abstract] [PDF]

				G. Wang, B. Wang, and J. Jiang Protein kinase A antagonizes Hedgehog signaling by regulating both the activator and repressor forms of Cubitus interruptus Genes & Dev., November 1, 1999; 13(21): 2828 - 2837. [Abstract] [Full Text]

				D. Stone, M Murone, S Luoh, W Ye, M. Armanini, A Gurney, H Phillips, J Brush, A Goddard, F. de Sauvage, et al. Characterization of the human suppressor of fused, a negative regulator of the zinc-finger transcription factor Gli J. Cell Sci., January 12, 1999; 112(23): 4437 - 4448. [Abstract] [PDF]

				J. T. Ohlmeyer and D. Kalderon Dual pathways for induction of wingless expression by protein kinase A and Hedgehog in Drosophila embryos Genes & Dev., September 1, 1997; 11(17): 2250 - 2258. [Abstract] [Full Text] [PDF]

				M. A. Stegman, J. E. Vallance, G. Elangovan, J. Sosinski, Y. Cheng, and D. J. Robbins Identification of a Tetrameric Hedgehog Signaling Complex J. Biol. Chem., July 14, 2000; 275(29): 21809 - 21812. [Abstract] [Full Text] [PDF]