- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Gilchrist, E. J.
- Articles by Moerman, D. G.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Gilchrist, E. J.
- Articles by Moerman, D. G.
Genetics, Vol 132, 431-442, Copyright © 1992
INVESTIGATIONS |
Mutations in the sup-38 Gene of Caenorhabditis elegans Suppress Muscle-Attachment Defects in unc-52 Mutants
E. J. Gilchrist and D. G. Moerman
Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 V6T 1Z4
Mutations in the unc-52 locus of Caenorhabditis elegans have been classified into three different groups based on their complex pattern of complementation. These mutations result in progressive paralysis (class 1 mutations) or in lethality (class 2 and 3 mutations). The paralysis exhibited by animals carrying class 1 mutations is caused by disruption of the myofilaments at their points of attachment to the cell membrane in the body wall muscle cells. We have determined that mutations of this class also have an effect on the somatic gonad, and this may be due to a similar disruption in the myoepithelial sheath cells of the uterus, or in the uterine muscle cells. Mutations that suppress the body wall muscle defects of the class 1 unc-52 mutations have been isolated, and they define a new locus, sup-38. Only the muscle disorganization of the Unc-52 mutants is suppressed; the gonad abnormalities are not, and the suppressors do not rescue the lethal phenotype of the class 2 and class 3 mutations. The suppressor mutations on their own exhibit a variable degree of gonad and muscle disorganization. Putative null sup-38 mutations cause maternal-effect lethality which is rescued by a wild-type copy of the locus in the zygote. These loss-of-function mutations have no effect on the body wall muscle structure.
This article has been cited by other articles:
 |
|
 |
|
  X. Jiang and J. R. Couchman Perlecan and Tumor Angiogenesis J. Histochem. Cytochem., November 1, 2003; 51(11): 1393 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Spike, A. G. Davies, J. E. Shaw, and R. K. Herman MEC-8 regulates alternative splicing of unc-52 transcripts in C. elegans hypodermal cells Development, January 11, 2002; 129(21): 4999 - 5008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Spike, J. E. Shaw, and R. K. Herman Analysis of smu-1, a Gene That Regulates the Alternative Splicing of unc-52 Pre-mRNA in Caenorhabditis elegans Mol. Cell. Biol., August 1, 2001; 21(15): 4985 - 4995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. P. Mullen, T. M. Rogalski, J. A. Bush, P. R. Gorji, and D. G. Moerman Complex Patterns of Alternative Splicing Mediate the Spatial and Temporal Distribution of Perlecan/UNC-52 in Caenorhabditis elegans Mol. Biol. Cell, October 1, 1999; 10(10): 3205 - 3221. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. G. Davies, C. A. Spike, J. E. Shaw, and R. K. Herman Functional Overlap Between the mec-8 Gene and Five sym Genes in Caenorhabditis elegans Genetics, September 1, 1999; 153(1): 117 - 134. [Abstract] [Full Text]
|
|
|
|
|
|
E. Lundquist, R. Herman, T. Rogalski, G. Mullen, D. Moerman, and J. Shaw The mec-8 gene of C. elegans encodes a protein with two RNA recognition motifs and regulates alternative splicing of unc-52 transcripts Development, January 5, 1996; 122(5): 1601 - 1610. [Abstract] [PDF]
|
|
|
|
 |
|
 T M Rogalski, B D Williams, G P Mullen, and D G Moerman Products of the unc-52 gene in Caenorhabditis elegans are homologous to the core protein of the mammalian basement membrane heparan sulfate proteoglycan. Genes & Dev., August 1, 1993; 7(8): 1471 - 1484. [Abstract] [PDF]
|
|