SPT4, SPT5 and SPT6 Interactions: Effects on Transcription and Viability in Saccharomyces cerevisiae

INVESTIGATIONS

SPT4, SPT5 and SPT6 Interactions: Effects on Transcription and Viability in Saccharomyces cerevisiae

M. S. Swanson and F. Winston
Current address: Department of Molecular Biology and Microbiology, Tufts University Medical School, Boston, Massachusetts 02111.

The SPT4, SPT5 and SPT6 genes of Saccharomyces cerevisiae were identified originally by mutations that suppress {delta} insertion mutations at HIS4 and LYS2. Subsequent analysis has demonstrated that spt4, spt5 and spt6 mutations confer similar pleiotropic phenotypes. They suppress {delta} insertion mutations by altering transcription and are believed to be required for normal transcription of several other loci. We have now analyzed interactions between SPT4, SPT5 and SPT6. First, the combination of mutations in any two of these three genes causes lethality in haploids. Second, some recessive mutations in different members of this set fail to complement each other. Third, mutations in all three genes alter transcription in similar ways. Finally, the results of coimmunoprecipitation experiments demonstate that at least the SPT5 and SPT6 proteins interact physically. Taken together, these genetic and biochemical results indicate that SPT4, SPT5 and SPT6 function together in a transcriptional process that is essential for viability in yeast.

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				D. Hess, B. Liu, N. R. Roan, R. Sternglanz, and F. Winston Spt10-Dependent Transcriptional Activation in Saccharomyces cerevisiae Requires both the Spt10 Acetyltransferase Domain and Spt21 Mol. Cell. Biol., January 1, 2004; 24(1): 135 - 143. [Abstract] [Full Text] [PDF]

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				S. C. Howard, A. Hester, and P. K. Herman The Ras/PKA Signaling Pathway May Control RNA Polymerase II Elongation via the Spt4p/Spt5p Complex in Saccharomyces cerevisiae Genetics, November 1, 2003; 165(3): 1059 - 1070. [Abstract] [Full Text] [PDF]

				M.-C. Keogh, V. Podolny, and S. Buratowski Bur1 Kinase Is Required for Efficient Transcription Elongation by RNA Polymerase II Mol. Cell. Biol., October 1, 2003; 23(19): 7005 - 7018. [Abstract] [Full Text] [PDF]

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				D. L. Lindstrom, S. L. Squazzo, N. Muster, T. A. Burckin, K. C. Wachter, C. A. Emigh, J. A. McCleery, J. R. Yates III, and G. A. Hartzog Dual Roles for Spt5 in Pre-mRNA Processing and Transcription Elongation Revealed by Identification of Spt5-Associated Proteins Mol. Cell. Biol., February 15, 2003; 23(4): 1368 - 1378. [Abstract] [Full Text] [PDF]

				J. A. Fischbeck, S. M. Kraemer, and L. A. Stargell SPN1, a Conserved Gene Identified by Suppression of a Postrecruitment-Defective Yeast TATA-Binding Protein Mutant Genetics, December 1, 2002; 162(4): 1605 - 1616. [Abstract] [Full Text] [PDF]

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				P.-Y. J. Wu and F. Winston Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex Mol. Cell. Biol., August 1, 2002; 22(15): 5367 - 5379. [Abstract] [Full Text] [PDF]

				B. R. Keegan, J. L. Feldman, D. H. Lee, D. S. Koos, R. K. Ho, D. Y. R. Stainier, and D. Yelon The elongation factors Pandora/Spt6 and Foggy/Spt5 promote transcription in the zebrafish embryo Development, January 4, 2002; 129(7): 1623 - 1632. [Abstract] [Full Text] [PDF]

				D. L. Lindstrom and G. A. Hartzog Genetic Interactions of Spt4-Spt5 and TFIIS With the RNA Polymerase II CTD and CTD Modifying Enzymes in Saccharomyces cerevisiae Genetics, October 1, 2001; 159(2): 487 - 497. [Abstract] [Full Text] [PDF]

				F. Malagon and A. Aguilera Yeast spt6-140 Mutation, Affecting Chromatin and Transcription, Preferentially Increases Recombination in Which Rad51p-Mediated Strand Exchange Is Dispensable Genetics, June 1, 2001; 158(2): 597 - 611. [Abstract] [Full Text] [PDF]

				P. J. Costa and K. M. Arndt Synthetic Lethal Interactions Suggest a Role for the Saccharomyces cerevisiae Rtf1 Protein in Transcription Elongation Genetics, October 1, 2000; 156(2): 535 - 547. [Abstract] [Full Text]

				M. Winkler, T. aus dem Siepen, and T. Stamminger Functional Interaction between Pleiotropic Transactivator pUL69 of Human Cytomegalovirus and the Human Homolog of Yeast Chromatin Regulatory Protein SPT6 J. Virol., September 1, 2000; 74(17): 8053 - 8064. [Abstract] [Full Text]

				J. K. Davie and C. M. Kane Genetic Interactions between TFIIS and the Swi-Snf Chromatin-Remodeling Complex Mol. Cell. Biol., August 15, 2000; 20(16): 5960 - 5973. [Abstract] [Full Text]

				Y. Hirose and J. L. Manley RNA polymerase II and the integration of nuclear events Genes & Dev., June 15, 2000; 14(12): 1415 - 1429. [Full Text]

				D. Ivanov, Y. T. Kwak, J. Guo, and R. B. Gaynor Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties Mol. Cell. Biol., May 1, 2000; 20(9): 2970 - 2983. [Abstract] [Full Text]

				M. Harata, Y. Oma, S. Mizuno, Y. W. Jiang, D. J. Stillman, and U. Wintersberger The Nuclear Actin-related Protein of Saccharomyces cerevisiae, Act3p/Arp4, Interacts with Core Histones Mol. Biol. Cell, August 1, 1999; 10(8): 2595 - 2605. [Abstract] [Full Text]

				Y. Wen and A. J. Shatkin Transcription elongation factor hSPT5 stimulates mRNA capping Genes & Dev., July 15, 1999; 13(14): 1774 - 1779. [Abstract] [Full Text]

				J. Stolz, U. Hoja, S. Meier, N. Sauer, and E. Schweizer Identification of the Plasma Membrane H+-Biotin Symporter of Saccharomyces cerevisiae by Rescue of a Fatty Acid-auxotrophic Mutant J. Biol. Chem., June 25, 1999; 274(26): 18741 - 18746. [Abstract] [Full Text] [PDF]

				Y. Yamaguchi, T. Wada, D. Watanabe, T. Takagi, J. Hasegawa, and H. Handa Structure and Function of the Human Transcription Elongation Factor DSIF J. Biol. Chem., March 19, 1999; 274(12): 8085 - 8092. [Abstract] [Full Text] [PDF]

				D. Donze, C. R. Adams, J. Rine, and R. T. Kamakaka The boundaries of the silenced HMR domain in Saccharomyces cerevisiae Genes & Dev., March 15, 1999; 13(6): 698 - 708. [Abstract] [Full Text]

				D. R. H. Evans, N. K. Brewster, Q. Xu, A. Rowley, B. A. Altheim, G. C. Johnston, and R. A. Singer The Yeast Protein Complex Containing Cdc68 and Pob3 Mediates Core-Promoter Repression Through the Cdc68 N-Terminal Domain Genetics, December 1, 1998; 150(4): 1393 - 1405. [Abstract] [Full Text]