- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Prival, M. J.
- Articles by Cebula, T. A.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Prival, M. J.
- Articles by Cebula, T. A.
Genetics, Vol 132, 303-310, Copyright © 1992
INVESTIGATIONS |
Sequence Analysis of Mutations Arising During Prolonged Starvation of Salmonella typhimurium
M. J. Prival and T. A. Cebula
Genetic Toxicology Branch Food and Drug Administration, Washington, D.C. 20204
We have examined the effects of prolonged histidine deprivation on the reversion of Salmonella typhimurium histidine auxotrophs containing either hisG46, a missense mutation (CTC -> CCC), or hisG428, an ochre mutation (CAA -> TAA). Both of these mutants can revert to His(+) via intragenic and extragenic mechanisms. Whereas the hisG46 mutant site consists of G/C base pairs, extragenic suppression of hisG46 requires mutation at an A/T site. Conversely, the hisG428 site itself contains only A/T base pairs, and extragenic suppression of hisG428 occurs principally at G/C sites. Thus, by examining the mutational spectrum of hisG46 and hisG428 revertants that occurred in the presence and in the absence of histidine, it was possible to determine the effects of histidine starvation on mutations at G/C vs. A/T sites as well as on intragenic sites vs. extragenic suppressor sites. Using DNA-colony hybridization, we determined the DNA sequences of over 1300 hisG46 and hisG428 revertants. Histidine-independent revertants that arose during growth in liquid medium that contained histidine included both intragenic and extragenic suppressor mutations. The relative frequency of such extragenic suppressors was greatly reduced among the His(+) revertants that were isolated after 5-10 days of histidine starvation on agar medium. Moreover, DNA sequence analysis revealed striking differences in the distribution of particular transversions at the hisG428 locus in revertants arising after prolonged histidine starvation as compared to those arising after growth in the presence of histidine.
This article has been cited by other articles:
 |
|
 |
|
  S. Porwollik, R. M.-Y. Wong, R. A. Helm, K. K. Edwards, M. Calcutt, A. Eisenstark, and M. McClelland DNA Amplification and Rearrangements in Archival Salmonella enterica Serovar Typhimurium LT2 Cultures J. Bacteriol., March 15, 2004; 186(6): 1678 - 1682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Saumaa, A. Tover, L. Kasak, and M. Kivisaar Different Spectra of Stationary-Phase Mutations in Early-Arising versus Late-Arising Mutants of Pseudomonas putida: Involvement of the DNA Repair Enzyme MutY and the Stationary-Phase Sigma Factor RpoS J. Bacteriol., December 15, 2002; 184(24): 6957 - 6965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Hudson, U. Bergthorsson, J. R. Roth, and H. Ochman Effect of Chromosome Location on Bacterial Mutation Rates Mol. Biol. Evol., January 1, 2002; 19(1): 85 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ohta, M. Watanabe-Akanuma, and H. Yamagata A comparison of mutation spectra detected by the Escherichia coli Lac+ reversion assay and the Salmonella typhimurium His+ reversion assay Mutagenesis, July 1, 2000; 15(4): 317 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Kasak, R. Horak, and M. Kivisaar Promoter-creating mutations in Pseudomonas putida: A model system for the study of mutation in starving bacteria PNAS, April 1, 1997; 94(7): 3134 - 3139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Foster and J. Trimarchi Adaptive reversion of a frameshift mutation in Escherichia coli by simple base deletions in homopolymeric runs Science, July 15, 1994; 265(5170): 407 - 409. [Abstract] [PDF]
|
|