- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Schofield, M. A.
- Articles by Miller, J. H.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Schofield, M. A.
- Articles by Miller, J. H.
Genetics, Vol 132, 295-302, Copyright © 1992
INVESTIGATIONS |
DNA Inversions Between Short Inverted Repeats in Escherichia coli
M. A. Schofield, R. Agbunag and J. H. Miller
Molecular Biology Institutes and Department of Microbiology and Molecular Genetics, University of California, Los Angeles, California 90024
Using site-specific mutagenesis in vitro, we have constructed Escherichia coli strains that allow the detection of the inversion of an 800-bp segment in the lac region. The invertible segment is bounded by inverted repeats of either 12 or 23 bp. Inversions occurring at these inverted repeats will restore the Lac(+) phenotype. Inversions can be detected at both short homologies at frequencies ranging from 0.5 X 10(-8) to 1 X 10(-7). These events, which have been verified by DNA sequence analysis, are reduced up to 1000-fold in strains deficient for either RecA, RecB or RecC. They are not reduced in strains deficient in the RecF,J pathway. These results show that the RecB,C,D system can mediate rearrangements at short sequence repeats, and probably plays a major role in cellular rearrangements.
This article has been cited by other articles:
 |
|
 |
|
  E. Kofoid, U. Bergthorsson, E. S. Slechta, and J. R. Roth Formation of an F' Plasmid by Recombination between Imperfectly Repeated Chromosomal Rep Sequences: a Closer Look at an Old Friend (F'128pro lac) J. Bacteriol., January 15, 2003; 185(2): 660 - 663. [Abstract] [Full Text] [PDF]
|
|