Use of a Yeast Site-Specific Recombinase to Produce Female Germline Chimeras in Drosophila

INVESTIGATIONS

Use of a Yeast Site-Specific Recombinase to Produce Female Germline Chimeras in Drosophila

T. B. Chou and N. Perrimon
Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115

We describe an efficient method for generating female germline mosaics by inducing site-specific homologous mitotic recombination with a yeast recombinase (FLP) which is driven by a heat shock promoter. These germline mosaics are produced in flies heterozygous for the agametic, germline-dependent, dominant female sterile (DFS) mutation ovo(D1), where only flies possessing germline clones are able to lay eggs. This method, the ``FLP-DFS'' technique, is very efficient because more than 90% of females with germline clones can be recovered. We show that this heat-inducible, site-specific mitotic recombination system does not affect viability and that the germline clones recovered are physiologically the same as those created by X-ray induced mitotic recombination. We describe the parameters of FLP-recombinase induced germline mitotic recombination and the use of the ``FLP-DFS'' technique to analyze the maternal effect of X-linked zygotic lethal mutations.

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