A Genetic Mapping System in Caenorhabditis elegans Based on Polymorphic Sequence-Tagged Sites

INVESTIGATIONS

A Genetic Mapping System in Caenorhabditis elegans Based on Polymorphic Sequence-Tagged Sites

B. D. Williams, B. Schrank, C. Huynh, R. Shownkeen and R. H. Waterston
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110

We devised an efficient genetic mapping system in the nematode Caenorhabditis elegans which is based upon the differences in number and location of the transposable element Tc1 between the Bristol and Bergerac strains. Using the nearly completed physical map of the C. elegans genome, we selected 40 widely distributed sites which contain a Tc1 element in the Bergerac strain, but not in the Bristol strain. For each site a polymerase chain reaction assay was designed that can distinguish between the Bergerac Tc1-containing site and the Bristol ``empty'' site. By combining appropriate assays in a single reaction, one can score multiple sites within single worms. This permits a mutation to be rapidly mapped, first to a linkage group and then to a chromosomal subregion, through analysis of only a small number of progeny from a single interstrain cross.

This article has been cited by other articles:

J. J. Bruinsma, D. L. Schneider, D. E. Davis, and K. Kornfeld
Identification of Mutations in Caenorhabditis elegans That Cause Resistance to High Levels of Dietary Zinc and Analysis Using a Genomewide Map of Single Nucleotide Polymorphisms Scored by Pyrosequencing
Genetics, June 1, 2008; 179(2): 811 - 828.
[Abstract] [Full Text] [PDF]

B. D. Galvin, S. Kim, and H. R. Horvitz
Caenorhabditis elegans Genes Required for the Engulfment of Apoptotic Corpses Function in the Cytotoxic Cell Deaths Induced by Mutations in lin-24 and lin-33
Genetics, May 1, 2008; 179(1): 403 - 417.
[Abstract] [Full Text] [PDF]

P. W. McDonald, S. L. Hardie, T. N. Jessen, L. Carvelli, D. S. Matthies, and R. D. Blakely
Vigorous Motor Activity in Caenorhabditis elegans Requires Efficient Clearance of Dopamine Mediated by Synaptic Localization of the Dopamine Transporter DAT-1
J. Neurosci., December 19, 2007; 27(51): 14216 - 14227.
[Abstract] [Full Text] [PDF]

A. Margalit, E. Neufeld, N. Feinstein, K. L. Wilson, B. Podbilewicz, and Y. Gruenbaum
Barrier to autointegration factor blocks premature cell fusion and maintains adult muscle integrity in C. elegans
J. Cell Biol., August 9, 2007; 178(4): 661 - 673.
[Abstract] [Full Text] [PDF]

K. Balamurugan, B. Ashokkumar, M. Moussaif, J. Y. Sze, and H. M. Said
Cloning and functional characterization of a folate transporter from the nematode Caenorhabditis elegans
Am J Physiol Cell Physiol, August 1, 2007; 293(2): C670 - C681.
[Abstract] [Full Text] [PDF]

C. M. Meighan and J. E. Schwarzbauer
Control of C. elegans hermaphrodite gonad size and shape by vab-3/Pax6-mediated regulation of integrin receptors
Genes & Dev., July 1, 2007; 21(13): 1615 - 1620.
[Abstract] [Full Text] [PDF]

S. Liegeois, A. Benedetto, G. Michaux, G. Belliard, and M. Labouesse
Genes Required for Osmoregulation and Apical Secretion in Caenorhabditis elegans
Genetics, February 1, 2007; 175(2): 709 - 724.
[Abstract] [Full Text] [PDF]

B. T. Carroll, G. R. Dubyak, M. M. Sedensky, and P. G. Morgan
Sulfated Signal from ASJ Sensory Neurons Modulates Stomatin-dependent Coordination in Caenorhabditis elegans
J. Biol. Chem., November 24, 2006; 281(47): 35989 - 35996.
[Abstract] [Full Text] [PDF]

L. Schaheen, G. Patton, and H. Fares
Suppression of the cup-5 mucolipidosis type IV-related lysosomal dysfunction by the inactivation of an ABC transporter in C. elegans
Development, October 1, 2006; 133(19): 3939 - 3948.
[Abstract] [Full Text] [PDF]

C. J. Ceol, F. Stegmeier, M. M. Harrison, and H. R. Horvitz
Identification and Classification of Genes That Act Antagonistically to let-60 Ras Signaling in Caenorhabditis elegans Vulval Development
Genetics, June 1, 2006; 173(2): 709 - 726.
[Abstract] [Full Text] [PDF]

I. Katic, L. G. Vallier, and I. Greenwald
New Positive Regulators of lin-12 Activity in Caenorhabditis elegans Include the BRE-5/Brainiac Glycosphingolipid Biosynthesis Enzyme
Genetics, December 1, 2005; 171(4): 1605 - 1615.
[Abstract] [Full Text] [PDF]

B. Goszczynski and J. D. McGhee
Reevaluation of the Role of the med-1 and med-2 Genes in Specifying the Caenorhabditis elegans Endoderm
Genetics, October 1, 2005; 171(2): 545 - 555.
[Abstract] [Full Text] [PDF]

T. Yabe, N. Suzuki, T. Furukawa, T. Ishihara, and I. Katsura
Multidrug resistance-associated protein MRP-1 regulates dauer diapause by its export activity in Caenorhabditis elegans
Development, July 15, 2005; 132(14): 3197 - 3207.
[Abstract] [Full Text] [PDF]

L. Yang, M. Sym, and C. Kenyon
The roles of two C. elegans HOX co-factor orthologs in cell migration and vulva development
Development, March 15, 2005; 132(6): 1413 - 1428.
[Abstract] [Full Text] [PDF]

M. Take-uchi, Y. Kobayashi, K. D. Kimura, T. Ishihara, and I. Katsura
FLR-4, a Novel Serine/Threonine Protein Kinase, Regulates Defecation Rhythm in Caenorhabditis elegans
Mol. Biol. Cell, March 1, 2005; 16(3): 1355 - 1365.
[Abstract] [Full Text] [PDF]

L. Granger, E. Martin, and L. Segalat
Mos as a tool for genome-wide insertional mutagenesis in Caenorhabditis elegans: results of a pilot study
Nucleic Acids Res., August 13, 2004; 32(14): e117 - e117.
[Abstract] [Full Text] [PDF]

A. K. Spartz, R. K. Herman, and J. E. Shaw
SMU-2 and SMU-1, Caenorhabditis elegans Homologs of Mammalian Spliceosome-Associated Proteins RED and fSAP57, Work Together To Affect Splice Site Choice
Mol. Cell. Biol., August 1, 2004; 24(15): 6811 - 6823.
[Abstract] [Full Text] [PDF]

G. R. Miley, D. Fantz, D. Glossip, X. Lu, R. M. Saito, R. E. Palmer, T. Inoue, S. van den Heuvel, P. W. Sternberg, and K. Kornfeld
Identification of Residues of the Caenorhabditis elegans LIN-1 ETS Domain That Are Necessary for DNA Binding and Regulation of Vulval Cell Fates
Genetics, August 1, 2004; 167(4): 1697 - 1709.
[Abstract] [Full Text] [PDF]

S. P. Koushika, A. M. Schaefer, R. Vincent, J. H. Willis, B. Bowerman, and M. L. Nonet
Mutations in Caenorhabditis elegans Cytoplasmic Dynein Components Reveal Specificity of Neuronal Retrograde Cargo
J. Neurosci., April 21, 2004; 24(16): 3907 - 3916.
[Abstract] [Full Text] [PDF]

N. Mehta, P. M. Loria, and O. Hobert
A Genetic Screen for Neurite Outgrowth Mutants in Caenorhabditis elegans Reveals a New Function for the F-box Ubiquitin Ligase Component LIN-23
Genetics, March 1, 2004; 166(3): 1253 - 1267.
[Abstract] [Full Text] [PDF]

E. Berezikov, C. I. Bargmann, and R. H. A. Plasterk
Homologous gene targeting in Caenorhabditis elegans by biolistic transformation
Nucleic Acids Res., February 24, 2004; 32(4): e40 - e40.
[Abstract] [Full Text] [PDF]

A. K. Burkeen, S. L. Maday, K. K. Rybicka, J. A. Sulcove, J. Ward, M. M. Huang, R. Barstead, C. Franzini-Armstrong,, and T. StC. Allen
Disruption of Caenorhabditis elegans Muscle Structure and Function Caused by Mutation of Troponin I
Biophys. J., February 1, 2004; 86(2): 991 - 1001.
[Abstract] [Full Text] [PDF]

D. C. Bergmann, M. Lee, B. Robertson, M.-F. B. Tsou, L. S. Rose, and W. B. Wood
Embryonic handedness choice in C. elegans involves the G{alpha} protein GPA-16
Development, December 1, 2003; 130(23): 5731 - 5740.
[Abstract] [Full Text] [PDF]

B. Roberts, C. Clucas, and I. L. Johnstone
Loss of SEC-23 in Caenorhabditis elegans Causes Defects in Oogenesis, Morphogenesis, and Extracellular Matrix Secretion
Mol. Biol. Cell, November 1, 2003; 14(11): 4414 - 4426.
[Abstract] [Full Text] [PDF]

Q. Ch'ng, L. Williams, Y. S. Lie, M. Sym, J. Whangbo, and C. Kenyon
Identification of Genes That Regulate a Left-Right Asymmetric Neuronal Migration in Caenorhabditis elegans
Genetics, August 1, 2003; 164(4): 1355 - 1367.
[Abstract] [Full Text] [PDF]

C.-c. Huang, D. H. Hall, E. M. Hedgecock, G. Kao, V. Karantza, B. E. Vogel, H. Hutter, A. D. Chisholm, P. D. Yurchenco, and W. G. Wadsworth
Laminin {alpha} subunits and their role in C. elegans development
Development, July 15, 2003; 130(14): 3343 - 3358.
[Abstract] [Full Text] [PDF]

S. Li, J. A. Dent, and R. Roy
Regulation of Intermuscular Electrical Coupling by the Caenorhabditis elegans Innexin inx-6
Mol. Biol. Cell, July 1, 2003; 14(7): 2630 - 2644.
[Abstract] [Full Text] [PDF]

J. H. Thomas, C. J. Ceol, H. T. Schwartz, and H. R. Horvitz
New Genes That Interact With lin-35 Rb to Negatively Regulate the let-60 ras Pathway in Caenorhabditis elegans
Genetics, May 1, 2003; 164(1): 135 - 151.
[Abstract] [Full Text] [PDF]

T. Hirose, Y. Nakano, Y. Nagamatsu, T. Misumi, H. Ohta, and Y. Ohshima
Cyclic GMP-dependent protein kinase EGL-4 controls body size and lifespan in C. elegans
Development, March 15, 2003; 130(6): 1089 - 1099.
[Abstract] [Full Text] [PDF]

S. Ayyadevara, R. Ayyadevara, A. Vertino, A. Galecki, J. J. Thaden, and R. J. S. Reis
Genetic Loci Modulating Fitness and Life Span in Caenorhabditis elegans: Categorical Trait Interval Mapping in CL2a x Bergerac-BO Recombinant-Inbred Worms
Genetics, February 1, 2003; 163(2): 557 - 570.
[Abstract] [Full Text] [PDF]

A. S.-R. Pepper, D. J. Killian, and E. J. A. Hubbard
Genetic Analysis of Caenorhabditis elegans glp-1 Mutants Suggests Receptor Interaction or Competition
Genetics, January 1, 2003; 163(1): 115 - 132.
[Abstract] [Full Text] [PDF]

P. M. Meneely, A. F. Farago, and T. M. Kauffman
Crossover Distribution and High Interference for Both the X Chromosome and an Autosome During Oogenesis and Spermatogenesis in Caenorhabditis elegans
Genetics, November 1, 2002; 162(3): 1169 - 1177.
[Abstract] [Full Text] [PDF]

A. J. MacQueen, M. P. Colaiacovo, K. McDonald, and A. M. Villeneuve
Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans
Genes & Dev., September 15, 2002; 16(18): 2428 - 2442.
[Abstract] [Full Text] [PDF]

K. A. Swan, D. E. Curtis, K. B. McKusick, A. V. Voinov, F. A. Mapa, and M. R. Cancilla
High-Throughput Gene Mapping in Caenorhabditis elegans
Genome Res., July 1, 2002; 12(7): 1100 - 1105.
[Abstract] [Full Text] [PDF]

S. E. Baird
Haldane's Rule by Sexual Transformation in Caenorhabditis
Genetics, July 1, 2002; 161(3): 1349 - 1353.
[Abstract] [Full Text] [PDF]

V. Hsu, C. L. Zobel, E. J. Lambie, T. Schedl, and K. Kornfeld
Caenorhabditis elegans lin-45 raf Is Essential for Larval Viability, Fertility and the Induction of Vulval Cell Fates
Genetics, February 1, 2002; 160(2): 481 - 492.
[Abstract] [Full Text] [PDF]

I. Kostic and R. Roy
Organ-specific cell division abnormalities caused by mutation in a general cell cycle regulator in C. elegans
Development, January 5, 2002; 129(9): 2155 - 2165.
[Abstract] [Full Text] [PDF]

K. Jia, P. S. Albert, and D. L. Riddle
DAF-9, a cytochrome P450 regulating C. elegans larval development and adult longevity
Development, January 1, 2002; 129(1): 221 - 231.
[Abstract] [Full Text] [PDF]

J. L. Schutzman, C. Z. Borland, J. C. Newman, M. K. Robinson, M. Kokel, and M. J. Stern
The Caenorhabditis elegans EGL-15 Signaling Pathway Implicates a DOS-Like Multisubstrate Adaptor Protein in Fibroblast Growth Factor Signal Transduction
Mol. Cell. Biol., December 1, 2001; 21(23): 8104 - 8116.
[Abstract] [Full Text] [PDF]

S. E. Basham and L. S. Rose
The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related protein of the AAA ATPase superfamily
Development, November 15, 2001; 128(22): 4645 - 4656.
[Abstract] [Full Text] [PDF]

H. Zhu, J. S. Duerr, H. Varoqui, J. R. McManus, J. B. Rand, and J. D. Erickson
Analysis of Point Mutants in the Caenorhabditis elegans Vesicular Acetylcholine Transporter Reveals Domains Involved in Substrate Translocation
J. Biol. Chem., November 2, 2001; 276(45): 41580 - 41587.
[Abstract] [Full Text] [PDF]

M. Boxem and S. van den Heuvel
lin-35 Rb and cki-1 Cip/Kip cooperate in developmental regulation of G1 progression in C. elegans
Development, November 1, 2001; 128(21): 4349 - 4359.
[Abstract] [Full Text] [PDF]

H. Fares and I. Greenwald
Genetic Analysis of Endocytosis in Caenorhabditis elegans: Coelomocyte Uptake Defective Mutants
Genetics, September 1, 2001; 159(1): 133 - 145.
[Abstract] [Full Text] [PDF]

C. A. Spike, J. E. Shaw, and R. K. Herman
Analysis of smu-1, a Gene That Regulates the Alternative Splicing of unc-52 Pre-mRNA in Caenorhabditis elegans
Mol. Cell. Biol., August 1, 2001; 21(15): 4985 - 4995.
[Abstract] [Full Text] [PDF]

A. J. MacQueen and A. M. Villeneuve
Nuclear reorganization and homologous chromosome pairing during meiotic prophase require C. elegans chk-2
Genes & Dev., July 1, 2001; 15(13): 1674 - 1687.
[Abstract] [Full Text] [PDF]

Q. H. Le, K. Turcotte, and T. Bureau
Tc8, a Tourist-like Transposon in Caenorhabditis elegans
Genetics, July 1, 2001; 158(3): 1081 - 1088.
[Abstract] [Full Text] [PDF]

Y. Zhang, B. Grant, and D. Hirsh
RME-8, a Conserved J-Domain Protein, Is Required for Endocytosis in Caenorhabditis elegans
Mol. Biol. Cell, July 1, 2001; 12(7): 2011 - 2021.
[Abstract] [Full Text] [PDF]

H. Du and M. Chalfie
Genes Regulating Touch Cell Development in Caenorhabditis elegans
Genetics, May 1, 2001; 158(1): 197 - 207.
[Abstract] [Full Text]

G. Shioi, M. Shoji, M. Nakamura, T. Ishihara, I. Katsura, H. Fujisawa, and S. Takagi
Mutations Affecting Nerve Attachment of Caenorhabditis elegans
Genetics, April 1, 2001; 157(4): 1611 - 1622.
[Abstract] [Full Text]

G. M. Chin and A. M. Villeneuve
C. elegans mre-11 is required for meiotic recombination and DNA repair but is dispensable for the meiotic G2 DNA damage checkpoint
Genes & Dev., March 1, 2001; 15(5): 522 - 534.
[Abstract] [Full Text]

V. Praitis, E. Casey, D. Collar, and J. Austin
Creation of Low-Copy Integrated Transgenic Lines in Caenorhabditis elegans
Genetics, March 1, 2001; 157(3): 1217 - 1226.
[Abstract] [Full Text]

S. Ayyadevara, R. Ayyadevara, S. Hou, J. J. Thaden, and R. J. S. Reis
Genetic Mapping of Quantitative Trait Loci Governing Longevity of Caenorhabditis elegans in Recombinant-Inbred Progeny of a Bergerac-BO RC301 Interstrain Cross
Genetics, February 1, 2001; 157(2): 655 - 666.
[Abstract] [Full Text]

S Alper and C Kenyon
REF-1, a protein with two bHLH domains, alters the pattern of cell fusion in C. elegans by regulating Hox protein activity
Development, January 5, 2001; 128(10): 1793 - 1804.
[Abstract] [PDF]

L. M. Miller, H. A. Hess, D. B. Doroquez, and N. M. Andrews
Null Mutations in the lin-31 Gene Indicate Two Functions During Caenorhabditis elegans Vulval Development
Genetics, December 1, 2000; 156(4): 1595 - 1602.
[Abstract] [Full Text]

J. Nance, E. B. Davis, and S. Ward
spe-29 Encodes a Small Predicted Membrane Protein Required for the Initiation of Sperm Activation in Caenorhabditis elegans
Genetics, December 1, 2000; 156(4): 1623 - 1633.
[Abstract] [Full Text]

D. M. Eisenmann and S. K. Kim
Protruding Vulva Mutants Identify Novel Loci and Wnt Signaling Factors That Function During Caenorhabditis elegans Vulva Development
Genetics, November 1, 2000; 156(3): 1097 - 1116.
[Abstract] [Full Text]

K. O. Kelly, A. F. Dernburg, G. M. Stanfield, and A. M. Villeneuve
Caenorhabditis elegans msh-5 Is Required for Both Normal and Radiation-Induced Meiotic Crossing Over but Not for Completion of Meiosis
Genetics, October 1, 2000; 156(2): 617 - 630.
[Abstract] [Full Text]

R. E. Kohn, J. S. Duerr, J. R. McManus, A. Duke, T. L. Rakow, H. Maruyama, G. Moulder, I. N. Maruyama, R. J. Barstead, and J. B. Rand
Expression of Multiple UNC-13 Proteins in the Caenorhabditis elegans Nervous System
Mol. Biol. Cell, October 1, 2000; 11(10): 3441 - 3452.
[Abstract] [Full Text]

R Clifford, M. Lee, S Nayak, M Ohmachi, F Giorgini, and T Schedl
FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the C. elegans hermaphrodite germline
Development, January 12, 2000; 127(24): 5265 - 5276.
[Abstract] [PDF]

L Molin, A Mounsey, S Aslam, P Bauer, J Young, M James, A Sharma-Oates, and I. Hope
Evolutionary conservation of redundancy between a diverged pair of forkhead transcription factor homologues
Development, January 11, 2000; 127(22): 4825 - 4835.
[Abstract] [PDF]

M. A. Lorson, H. R. Horvitz, and S. van den Heuvel
LIN-5 Is a Novel Component of the Spindle Apparatus Required for Chromosome Segregation and Cleavage Plane Specification in Caenorhabditis elegans
J. Cell Biol., January 10, 2000; 148(1): 73 - 86.
[Abstract] [Full Text] [PDF]

M. Jeon, H. F. Gardner, E. A. Miller, J. Deshler, and A. E. Rougvie
Similarity of the C. elegans Developmental Timing Protein LIN-42 to Circadian Rhythm Proteins
Science, November 5, 1999; 286(5442): 1141 - 1146.
[Abstract] [Full Text]

A. R. Winnier, J. Y.-J. Meir, J. M. Ross, N. Tavernarakis, M. Driscoll, T. Ishihara, I. Katsura, and D. M. Miller III
UNC-4/UNC-37-dependent repression of motor neuron-specific genes controls synaptic choice in Caenorhabditis elegans
Genes & Dev., November 1, 1999; 13(21): 2774 - 2786.
[Abstract] [Full Text]

				B. D. Galvin, S. Kim, and H. R. Horvitz Caenorhabditis elegans Genes Required for the Engulfment of Apoptotic Corpses Function in the Cytotoxic Cell Deaths Induced by Mutations in lin-24 and lin-33 Genetics, May 1, 2008; 179(1): 403 - 417. [Abstract] [Full Text] [PDF]

				P. W. McDonald, S. L. Hardie, T. N. Jessen, L. Carvelli, D. S. Matthies, and R. D. Blakely Vigorous Motor Activity in Caenorhabditis elegans Requires Efficient Clearance of Dopamine Mediated by Synaptic Localization of the Dopamine Transporter DAT-1 J. Neurosci., December 19, 2007; 27(51): 14216 - 14227. [Abstract] [Full Text] [PDF]

				A. Margalit, E. Neufeld, N. Feinstein, K. L. Wilson, B. Podbilewicz, and Y. Gruenbaum Barrier to autointegration factor blocks premature cell fusion and maintains adult muscle integrity in C. elegans J. Cell Biol., August 9, 2007; 178(4): 661 - 673. [Abstract] [Full Text] [PDF]

				K. Balamurugan, B. Ashokkumar, M. Moussaif, J. Y. Sze, and H. M. Said Cloning and functional characterization of a folate transporter from the nematode Caenorhabditis elegans Am J Physiol Cell Physiol, August 1, 2007; 293(2): C670 - C681. [Abstract] [Full Text] [PDF]

				C. M. Meighan and J. E. Schwarzbauer Control of C. elegans hermaphrodite gonad size and shape by vab-3/Pax6-mediated regulation of integrin receptors Genes & Dev., July 1, 2007; 21(13): 1615 - 1620. [Abstract] [Full Text] [PDF]

				S. Liegeois, A. Benedetto, G. Michaux, G. Belliard, and M. Labouesse Genes Required for Osmoregulation and Apical Secretion in Caenorhabditis elegans Genetics, February 1, 2007; 175(2): 709 - 724. [Abstract] [Full Text] [PDF]

				B. T. Carroll, G. R. Dubyak, M. M. Sedensky, and P. G. Morgan Sulfated Signal from ASJ Sensory Neurons Modulates Stomatin-dependent Coordination in Caenorhabditis elegans J. Biol. Chem., November 24, 2006; 281(47): 35989 - 35996. [Abstract] [Full Text] [PDF]

				L. Schaheen, G. Patton, and H. Fares Suppression of the cup-5 mucolipidosis type IV-related lysosomal dysfunction by the inactivation of an ABC transporter in C. elegans Development, October 1, 2006; 133(19): 3939 - 3948. [Abstract] [Full Text] [PDF]

				C. J. Ceol, F. Stegmeier, M. M. Harrison, and H. R. Horvitz Identification and Classification of Genes That Act Antagonistically to let-60 Ras Signaling in Caenorhabditis elegans Vulval Development Genetics, June 1, 2006; 173(2): 709 - 726. [Abstract] [Full Text] [PDF]

				I. Katic, L. G. Vallier, and I. Greenwald New Positive Regulators of lin-12 Activity in Caenorhabditis elegans Include the BRE-5/Brainiac Glycosphingolipid Biosynthesis Enzyme Genetics, December 1, 2005; 171(4): 1605 - 1615. [Abstract] [Full Text] [PDF]

				B. Goszczynski and J. D. McGhee Reevaluation of the Role of the med-1 and med-2 Genes in Specifying the Caenorhabditis elegans Endoderm Genetics, October 1, 2005; 171(2): 545 - 555. [Abstract] [Full Text] [PDF]

				T. Yabe, N. Suzuki, T. Furukawa, T. Ishihara, and I. Katsura Multidrug resistance-associated protein MRP-1 regulates dauer diapause by its export activity in Caenorhabditis elegans Development, July 15, 2005; 132(14): 3197 - 3207. [Abstract] [Full Text] [PDF]

				L. Yang, M. Sym, and C. Kenyon The roles of two C. elegans HOX co-factor orthologs in cell migration and vulva development Development, March 15, 2005; 132(6): 1413 - 1428. [Abstract] [Full Text] [PDF]

				M. Take-uchi, Y. Kobayashi, K. D. Kimura, T. Ishihara, and I. Katsura FLR-4, a Novel Serine/Threonine Protein Kinase, Regulates Defecation Rhythm in Caenorhabditis elegans Mol. Biol. Cell, March 1, 2005; 16(3): 1355 - 1365. [Abstract] [Full Text] [PDF]

				L. Granger, E. Martin, and L. Segalat Mos as a tool for genome-wide insertional mutagenesis in Caenorhabditis elegans: results of a pilot study Nucleic Acids Res., August 13, 2004; 32(14): e117 - e117. [Abstract] [Full Text] [PDF]

				A. K. Spartz, R. K. Herman, and J. E. Shaw SMU-2 and SMU-1, Caenorhabditis elegans Homologs of Mammalian Spliceosome-Associated Proteins RED and fSAP57, Work Together To Affect Splice Site Choice Mol. Cell. Biol., August 1, 2004; 24(15): 6811 - 6823. [Abstract] [Full Text] [PDF]

				G. R. Miley, D. Fantz, D. Glossip, X. Lu, R. M. Saito, R. E. Palmer, T. Inoue, S. van den Heuvel, P. W. Sternberg, and K. Kornfeld Identification of Residues of the Caenorhabditis elegans LIN-1 ETS Domain That Are Necessary for DNA Binding and Regulation of Vulval Cell Fates Genetics, August 1, 2004; 167(4): 1697 - 1709. [Abstract] [Full Text] [PDF]

				S. P. Koushika, A. M. Schaefer, R. Vincent, J. H. Willis, B. Bowerman, and M. L. Nonet Mutations in Caenorhabditis elegans Cytoplasmic Dynein Components Reveal Specificity of Neuronal Retrograde Cargo J. Neurosci., April 21, 2004; 24(16): 3907 - 3916. [Abstract] [Full Text] [PDF]

				N. Mehta, P. M. Loria, and O. Hobert A Genetic Screen for Neurite Outgrowth Mutants in Caenorhabditis elegans Reveals a New Function for the F-box Ubiquitin Ligase Component LIN-23 Genetics, March 1, 2004; 166(3): 1253 - 1267. [Abstract] [Full Text] [PDF]

				E. Berezikov, C. I. Bargmann, and R. H. A. Plasterk Homologous gene targeting in Caenorhabditis elegans by biolistic transformation Nucleic Acids Res., February 24, 2004; 32(4): e40 - e40. [Abstract] [Full Text] [PDF]

				A. K. Burkeen, S. L. Maday, K. K. Rybicka, J. A. Sulcove, J. Ward, M. M. Huang, R. Barstead, C. Franzini-Armstrong,, and T. StC. Allen Disruption of Caenorhabditis elegans Muscle Structure and Function Caused by Mutation of Troponin I Biophys. J., February 1, 2004; 86(2): 991 - 1001. [Abstract] [Full Text] [PDF]

				D. C. Bergmann, M. Lee, B. Robertson, M.-F. B. Tsou, L. S. Rose, and W. B. Wood Embryonic handedness choice in C. elegans involves the G{alpha} protein GPA-16 Development, December 1, 2003; 130(23): 5731 - 5740. [Abstract] [Full Text] [PDF]

				B. Roberts, C. Clucas, and I. L. Johnstone Loss of SEC-23 in Caenorhabditis elegans Causes Defects in Oogenesis, Morphogenesis, and Extracellular Matrix Secretion Mol. Biol. Cell, November 1, 2003; 14(11): 4414 - 4426. [Abstract] [Full Text] [PDF]

				Q. Ch'ng, L. Williams, Y. S. Lie, M. Sym, J. Whangbo, and C. Kenyon Identification of Genes That Regulate a Left-Right Asymmetric Neuronal Migration in Caenorhabditis elegans Genetics, August 1, 2003; 164(4): 1355 - 1367. [Abstract] [Full Text] [PDF]

				C.-c. Huang, D. H. Hall, E. M. Hedgecock, G. Kao, V. Karantza, B. E. Vogel, H. Hutter, A. D. Chisholm, P. D. Yurchenco, and W. G. Wadsworth Laminin {alpha} subunits and their role in C. elegans development Development, July 15, 2003; 130(14): 3343 - 3358. [Abstract] [Full Text] [PDF]

				S. Li, J. A. Dent, and R. Roy Regulation of Intermuscular Electrical Coupling by the Caenorhabditis elegans Innexin inx-6 Mol. Biol. Cell, July 1, 2003; 14(7): 2630 - 2644. [Abstract] [Full Text] [PDF]

				J. H. Thomas, C. J. Ceol, H. T. Schwartz, and H. R. Horvitz New Genes That Interact With lin-35 Rb to Negatively Regulate the let-60 ras Pathway in Caenorhabditis elegans Genetics, May 1, 2003; 164(1): 135 - 151. [Abstract] [Full Text] [PDF]

				T. Hirose, Y. Nakano, Y. Nagamatsu, T. Misumi, H. Ohta, and Y. Ohshima Cyclic GMP-dependent protein kinase EGL-4 controls body size and lifespan in C. elegans Development, March 15, 2003; 130(6): 1089 - 1099. [Abstract] [Full Text] [PDF]

				S. Ayyadevara, R. Ayyadevara, A. Vertino, A. Galecki, J. J. Thaden, and R. J. S. Reis Genetic Loci Modulating Fitness and Life Span in Caenorhabditis elegans: Categorical Trait Interval Mapping in CL2a x Bergerac-BO Recombinant-Inbred Worms Genetics, February 1, 2003; 163(2): 557 - 570. [Abstract] [Full Text] [PDF]

				A. S.-R. Pepper, D. J. Killian, and E. J. A. Hubbard Genetic Analysis of Caenorhabditis elegans glp-1 Mutants Suggests Receptor Interaction or Competition Genetics, January 1, 2003; 163(1): 115 - 132. [Abstract] [Full Text] [PDF]