- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Ross, L. O.
- Articles by Dawson, D.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Ross, L. O.
- Articles by Dawson, D.
Genetics, Vol 131, 541-550, Copyright © 1992
INVESTIGATIONS |
Meiotic Recombination on Artificial Chromosomes in Yeast
L. O. Ross, D. Treco, A. Nicolas, J. W. Szostak and D. Dawson
Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts 02111
We have examined the meiotic recombination characteristics of artificial chromosomes in Saccharomyces cerevisiae. Our experiments were carried out using minichromosome derivatives of yeast chromosome III and yeast artificial chromosomes composed primarily of bacteriophage {lambda} DNA. Tetrad analysis revealed that the artificial chromosomes exhibit very low levels of meiotic recombination. However, when a 12.5-kbp fragment from yeast chromosome VIII was inserted into the right arm of the artificial chromosome, recombination within that arm mimicked the recombination characteristics of the fragment in its natural context including the ability of crossovers to ensure meiotic disjunction. Both crossing over and gene conversion (within the ARG4 gene contained within the fragment) were measured in the experiments. Similarly, a 55-kbp region from chromosome III carried on a minichromosome showed crossover behavior indistinguishable from that seen when it is carried on chromosome III. We discuss the notion that, in yeast, meiotic recombination behavior is determined locally by small chromosomal regions that function free of the influence of the chromosome as a whole.
This article has been cited by other articles:
 |
|
 |
|
  B. Kemp, R. M. Boumil, M. N. Stewart, and D. S. Dawson A role for centromere pairing in meiotic chromosome segregation Genes & Dev., August 15, 2004; 18(16): 1946 - 1951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Haring, G. R. Halley, A. J. Jones, and R. E. Malone Properties of Natural Double-Strand-Break Sites at a Recombination Hotspot in Saccharomyces cerevisiae Genetics, September 1, 2003; 165(1): 101 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Krawchuk and W. P. Wahls Centromere Mapping Functions for Aneuploid Meiotic Products: Analysis of rec8, rec10 and rec11 Mutants of the Fission Yeast Schizosaccharomyces pombe Genetics, September 1, 1999; 153(1): 49 - 55. [Abstract] [Full Text]
|
|