- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Masison, D. C.
- Articles by Baker, R. E.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Masison, D. C.
- Articles by Baker, R. E.
Genetics, Vol 131, 43-53, Copyright © 1992
INVESTIGATIONS |
Meiosis in Saccharomyces cerevisiae Mutants Lacking the Centromere-Binding Protein CP1
D. C. Masison and R. E. Baker
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655
CP1 (encoded by the CEP1 gene) is a centromere binding protein of Saccharomyces cerevisiae that binds to the conserved DNA element I (CDEI) of yeast centromeres. To investigate the function of CP1 in yeast meiosis, we analyzed the meiotic segregation of CEN plasmids, nonessential chromosome fragments (CFs) and chromosomes in cep1 null mutants. Plasmids and CFs missegregated in 10-20% of meioses with the most frequent type of aberrant event being precocious sister segregation at the first meiotic division; paired and unpaired CFs behaved similarly. An unpaired chromosome I homolog (2N + 1) also missegregated at high frequency in the cep1 mutant (7.6%); however, missegregation of other chromosomes was not detected by tetrad analysis. Spore viability of cep1 tetrads was significantly reduced, and the pattern of spore death was nonrandom. The inviability could not be explained solely by chromosome missegregation and is probably a pleiotropic effect of cep1. Mitotic chromosome loss in cep1 strains was also analyzed. Both simple loss (1:0 segregation) and nondisjunction (2:0 segregation) were increased, but the majority of loss events resulted from nondisjunction. We interpret the results to suggest that CP1 generally promotes chromatid-kinetochore adhesion.
This article has been cited by other articles:
 |
|
 |
|
  N. A. Kent, S. M. Eibert, and J. Mellor Cbf1p Is Required for Chromatin Remodeling at Promoter-proximal CACGTG Motifs in Yeast J. Biol. Chem., June 25, 2004; 279(26): 27116 - 27123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zeng and W. S. Saunders The Saccharomyces cerevisiae Centromere Protein Slk19p Is Required for Two Successive Divisions During Meiosis Genetics, June 1, 2000; 155(2): 577 - 587. [Abstract] [Full Text]
|
|
|
|
|
|
R. E. Baker, K. Harris, and K. Zhang Mutations Synthetically Lethal with cep1 Target S. cerevisiae Kinetochore Components Genetics, May 1, 1998; 149(1): 73 - 85. [Abstract] [Full Text] [PDF]
|
|