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Genetics, Vol 130, 865-872, Copyright © 1992
INVESTIGATIONS |
Synaptonemal Complexes from DNase-Treated Rat Pachytene Chromosomes Contain (GT)(n) and LINE/SINE Sequences
R. E. Pearlman, N. Tsao and P. B. Moens
Department of Biology, York University, Downsview, Ontario, Canada M3J 1P3
Purified chromosome cores (synaptonemal complexes) of rat pachytene chromosomes, from which the chromatin is removed by extensive DNase II digestion, retain a residual class of DNA, presumably the bases of chromatin loops. This synaptonemal complex-associated DNA, isolated by proteinase digestion and phenol extraction of purified DNase-treated synaptonemal complexes, and cloned in plasmid vector pEMBL18, has a length distribution of 50-500 bp. From a library of these fragments, 21 fragments were sequenced. Present in this sample are short 40-200-bp segments with >80% identity to ``long'' and ``short'' interspersed repeated elements (LINE/SINEs), an excess of GT/CA tandem repeats and a number of unidentified sequences. The LINE/SINE segments may play a role in homology vs. nonhomology recognition during meiosis and the alternating purine-pyrimidine sequences have been implicated in genetic recombination. Their enrichment in synaptonemal complexes may be related to the synapsis and recombination functions of meiosis.
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