- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Garfinkel, M. D.
- Articles by Mahowald, A. P.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Garfinkel, M. D.
- Articles by Mahowald, A. P.
Genetics, Vol 130, 791-803, Copyright © 1992
INVESTIGATIONS |
Molecular Genetics of the Drosophila melanogaster ovo Locus, a Gene Required for Sex Determination of Germline Cells
M. D. Garfinkel, A. R. Lohe and A. P. Mahowald
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, and Department of Genetics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 Present address: Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637.
The Drosophila melanogaster ovo gene is required for survival and differentiation of female germline cells, apparently playing a role in germline sex determination. We recovered 60 kb of genomic DNA from its genetic location at 4E1,2 on the X chromosome. A transcription unit coding for an apparently female-specific germline-dependent 5-kb poly(A)(+) RNA size class is located substantially in a 7-kb region, within which three DNA-detectable lesions for mutations that inactivate the ovo function are located at two sites {complex}4 kb apart. The breakpoint of a deficiency that removes the neighboring lethal complementation group shavenbaby (svb) but leaves the ovo function intact maps {complex}5 kb to the molecular left of the leftmost ovo mutant site. A class of mutations that inactivates both the svb function and the ovo function affects genomic DNA between the two ovo sites. Sequences required for the two genetic functions are partly overlapping. In spite of this overlap, P element-mediated gene transfer of a 10-kb genomic DNA segment containing the 5-kb poly(A)(+) RNA transcription unit rescues the female sterility phenotypes of ovo mutations, but not the svb lethality.
This article has been cited by other articles:
 |
|
 |
|
  A. Casper and M. Van Doren The control of sexual identity in the Drosophila germline Development, August 1, 2006; 133(15): 2783 - 2791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Bielinska, J. Lu, D. Sturgill, and B. Oliver Core Promoter Sequences Contribute to ovo-B Regulation in the Drosophila melanogaster Germline Genetics, January 1, 2005; 169(1): 161 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Mandon-Pepin, A. Oustry-Vaiman, B. Vigier, F. Piumi, E. Cribiu, and C. Cotinot Expression Profiles and Chromosomal Localization of Genes Controlling Meiosis and Follicular Development in the Sheep Ovary Biol Reprod, March 1, 2003; 68(3): 985 - 995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Labrador and V. G. Corces Protein Determinants of Insertional Specificity for the Drosophila Gypsy Retrovirus Genetics, July 1, 2001; 158(3): 1101 - 1110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lee and M. D. Garfinkel Characterization of Drosophila OVO protein DNA binding specificity using random DNA oligomer selection suggests zinc finger degeneration Nucleic Acids Res., February 1, 2000; 28(3): 826 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Andrews, D Garcia-Estefania, I Delon, J Lu, M Mevel-Ninio, A Spierer, F Payre, D Pauli, and B Oliver OVO transcription factors function antagonistically in the Drosophila female germline Development, January 2, 2000; 127(4): 881 - 892. [Abstract] [PDF]
|
|
|
|
 |
|
 X. Dai, C. Schonbaum, L. Degenstein, W. Bai, A. Mahowald, and E. Fuchs The ovo gene required for cuticle formation and oogenesis in flies is involved in hair formation and spermatogenesis in mice Genes & Dev., November 1, 1998; 12(21): 3452 - 3463. [Abstract] [Full Text]
|
|
|
|
|
|
M Mevel-Ninio, E Fouilloux, I Guenal, and A Vincent The three dominant female-sterile mutations of the Drosophila ovo gene are point mutations that create new translation-initiator AUG codons Development, January 12, 1996; 122(12): 4131 - 4138. [Abstract] [PDF]
|
|
|
|
|
|
B Oliver, J Singer, V Laget, G Pennetta, and D Pauli Function of Drosophila ovo+ in germ-line sex determination depends on X-chromosome number Development, January 11, 1994; 120(11): 3185 - 3195. [Abstract] [PDF]
|
|
|
|
|
|
T. B. Chou, E. Noll, and N. Perrimon Autosomal P[ovoD1] dominant female-sterile insertions in Drosophila and their use in generating germ-line chimeras Development, December 1, 1993; 119(4): 1359 - 1369. [Abstract] [PDF]
|
|