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Genetics, Vol 130, 729-736, Copyright © 1992
INVESTIGATIONS |
Use of Gene Replacement Transformation to Elucidate Gene Function in the qa Gene Cluster of Neurospora crassa
M. E. Case, R. F. Geever and D. K. Asch
Department of Genetics, University of Georgia, Athens, GA 30602
Gene replacement by transformation, employing selective genetic recombination techniques, has been used to delete or disrupt the qa-x, qa-y and qa-1S genes of the qa gene cluster of Neurospora crassa. The growth characteristics of the strain carrying the deletion of the qa-y gene support earlier evidence that this gene encodes a quinic acid permease. The strain containing the deletion of the qa-1S gene ({Delta}qa-1S) was examined with respect to quinic acid induction and carbon catabolite repression. The {Delta}qa-1S strain exhibits constitutive expression of the qa genes supporting earlier evidence that the qa-1S gene codes for a repressor. Several of the qa genes continued to be expressed at high levels even in the presence of glucose in the {Delta}qa-1S strain, which indicates that transcription of these genes is not being affected directly by a repressor molecule in the presence of glucose.
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