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Genetics, Vol 130, 295-304, Copyright © 1992
INVESTIGATIONS |
Analysis of URS(G)-Mediated Glucose Repression of the GAL1 Promoter of Saccharomyces cerevisiae
J. S. Flick and M. Johnston
Department of Genetics, Washington University Medical School, St. Louis, Missouri 63110
Repression of GAL1 expression during growth on glucose is mediated in part by cis-acting promoter elements designated URS(G). We show that oligonucleotides containing sequences from two regions of URS(G) confer glucose repression upon a heterologous promoter. Repression caused by URS(G) is dependent on trans-acting factors of the glucose repression pathway and is independent of orientation or location within a promoter, suggesting that URS(G) contains binding sites for a glucose-activated repressor protein(s). Genetic analysis identified three apparently novel genes (URR1, URR3 and URR4) that are specifically required for URS(G)-mediated repression and may encode such repressor proteins. Mutations in the URR genes suppress the defect in URS(G) derepression caused by a snf1 mutation.
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