Seventeen Complementation Groups of Mutations Decreasing Meiotic Recombination in Schizosaccharomyces pombe

INVESTIGATIONS

Seventeen Complementation Groups of Mutations Decreasing Meiotic Recombination in Schizosaccharomyces pombe

L. C. DeVeaux, N. A. Hoagland and G. R. Smith
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104

We have analyzed 43 recessive mutations reducing meiotic intragenic recombination in Schizosaccharomyces pombe. These mutations were isolated by a screen for reduced plasmid-by-chromosome recombination at the ade6 locus. Sixteen of the mutations define 10 new complementation groups, bringing to 17 the number of genes identified to be involved in meiotic recombination. The mutations were grouped into three discrete classes depending on the severity of the recombination deficiency in crosses involving the ade6-M26 recombination hotspot. Class I mutations caused at least a 1000-fold reduction in M26-stimulated intragenic recombination at the ade6 locus. Class II mutations reduced M26-stimulated recombination approximately 100-fold. Class III mutations caused a 3-10-fold reduction in either M26-stimulated or non-hotspot recombination. We obtained multiple alleles of class I and class II mutations, suggesting that we may be nearing saturation for mutations of this type. As a first step toward mapping, we used mitotic segregation to assign fourteen of the rec genes to chromosomes. Mutations in the six rec genes tested also caused a decrease in intragenic recombination at the ura4 locus; five of these mutations also reduced intergenic recombination between the pro2 and arg3 genes. These results indicate that these multiple rec gene products are required for high level meiotic recombination throughout the S. pombe genome.

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				C. Ellermeier and G. R. Smith Cohesins are required for meiotic DNA breakage and recombination in Schizosaccharomyces pombe PNAS, August 2, 2005; 102(31): 10952 - 10957. [Abstract] [Full Text] [PDF]

				L. Davis and G. R. Smith Dynein Promotes Achiasmate Segregation in Schizosaccharomyces pombe Genetics, June 1, 2005; 170(2): 581 - 590. [Abstract] [Full Text] [PDF]

				D. W. Pryce, A. Lorenz, J. B. Smirnova, J. Loidl, and R. J. McFarlane Differential Activation of M26-Containing Meiotic Recombination Hot Spots in Schizosaccharomyces pombe Genetics, May 1, 2005; 170(1): 95 - 106. [Abstract] [Full Text] [PDF]

				J. A. Farah, G. Cromie, W. W. Steiner, and G. R. Smith A Novel Recombination Pathway Initiated by the Mre11/Rad50/Nbs1 Complex Eliminates Palindromes During Meiosis in Schizosaccharomyces pombe Genetics, March 1, 2005; 169(3): 1261 - 1274. [Abstract] [Full Text] [PDF]

				G. A. Cromie, C. A. Rubio, R. W. Hyppa, and G. R. Smith A Natural Meiotic DNA Break Site in Schizosaccharomyces pombe Is a Hotspot of Gene Conversion, Highly Associated With Crossing Over Genetics, February 1, 2005; 169(2): 595 - 605. [Abstract] [Full Text] [PDF]

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				M. E. Fox, T. Yamada, K. Ohta, and G. R. Smith A Family of cAMP-Response-Element-Related DNA Sequences With Meiotic Recombination Hotspot Activity in Schizosaccharomyces pombe Genetics, September 1, 2000; 156(1): 59 - 68. [Abstract] [Full Text]

				M. D. Krawchuk and W. P. Wahls Centromere Mapping Functions for Aneuploid Meiotic Products: Analysis of rec8, rec10 and rec11 Mutants of the Fission Yeast Schizosaccharomyces pombe Genetics, September 1, 1999; 153(1): 49 - 55. [Abstract] [Full Text]

				M. D. Krawchuk, L. C. DeVeaux, and W. P. Wahls Meiotic Chromosome Dynamics Dependent Upon the rec8+, rec10+ and rec11+ Genes of the Fission Yeast Schizosaccharomyces pombe Genetics, September 1, 1999; 153(1): 57 - 68. [Abstract] [Full Text]

				S. Parisi, M. J. McKay, M. Molnar, M. A. Thompson, P. J. van der Spek, E. van Drunen-Schoenmaker, R. Kanaar, E. Lehmann, J. H. J. Hoeijmakers, and J. Kohli Rec8p, a Meiotic Recombination and Sister Chromatid Cohesion Phosphoprotein of the Rad21p Family Conserved from Fission Yeast to Humans Mol. Cell. Biol., May 1, 1999; 19(5): 3515 - 3528. [Abstract] [Full Text] [PDF]

				N. Kon, S. C. Schroeder, M. D. Krawchuk, and W. P. Wahls Regulation of the Mts1-Mts2-Dependent ade6-M26 Meiotic Recombination Hot Spot and Developmental Decisions by the Spc1 Mitogen-Activated Protein Kinase of Fission Yeast Mol. Cell. Biol., December 1, 1998; 18(12): 7575 - 7583. [Abstract] [Full Text]

				J. E. Masson and J. Paszkowski Arabidopsis thaliana mutants altered in homologous recombination PNAS, October 14, 1997; 94(21): 11731 - 11735. [Abstract] [Full Text] [PDF]

				M. E. Fox, J. B. Virgin, J. Metzger, and G. R. Smith Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 PNAS, July 8, 1997; 94(14): 7446 - 7451. [Abstract] [Full Text] [PDF]

				P Szankasi and G. Smith A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction Science, February 24, 1995; 267(5201): 1166 - 1169. [Abstract] [PDF]

				W P Wahls and G R Smith A heteromeric protein that binds to a meiotic homologous recombination hot spot: correlation of binding and hot spot activity. Genes & Dev., July 15, 1994; 8(14): 1693 - 1702. [Abstract] [PDF]

				L C DeVeaux and G R Smith Region-specific activators of meiotic recombination in Schizosaccharomyces pombe. Genes & Dev., January 15, 1994; 8(2): 203 - 210. [Abstract] [PDF]