- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Komoda, Y.
- Articles by Tominaga, A.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Komoda, Y.
- Articles by Tominaga, A.
Genetics, Vol 129, 639-645, Copyright © 1991
INVESTIGATIONS |
Large Inversion in Escherichia coli K-12 1485IN Between Inversely Oriented IS3 Elements Near lac and cdd
Y. Komoda, M. Enomoto and A. Tominaga
Department of Biology, Faculty of Science, Okayama University, Okayama 700, Japan
A companion study has shown that the inversion carried by strain 1485IN has one terminus between lac and proC and the other between his and cdd of the normal strain. Starting with this mapping data, we have done molecular work demonstrating that the inversion occurred by recombination between inversely oriented two IS3 elements, one present near lac and the other near the cdd locus; i.e., the inversion is IN(is3B-is3E). Evidence supporting this conclusion includes: (i) Normal and inversion strains share two short regions with identical restriction maps. One of these regions is near lac and the other near cdd. (ii) IS3 homology was detected in each of the terminus regions of both the normal and inversion strains. (iii) The sequence on one side of the original IS3 element near lac has been exchanged with the sequence on one side of the IS3 near cdd. Whether the inversion has occurred by one event of homologous recombination between the two IS3 elements or has been caused by involvement of IS3 elements on an F factor is discussed. Another rearrangement, probably related to inversion and deletion, was detected between the IS3 and cdd of the inversion strain.
This article has been cited by other articles:
 |
|
 |
|
  M.-L. Daveran-Mingot, N. Campo, P. Ritzenthaler, and P. Le Bourgeois A Natural Large Chromosomal Inversion in Lactococcus lactis Is Mediated by Homologous Recombination between Two Insertion Sequences J. Bacteriol., September 15, 1998; 180(18): 4834 - 4842. [Abstract] [Full Text]
|
|