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Genetics, Vol 129, 501-512, Copyright © 1991
INVESTIGATIONS |
The Maternally Inherited Regulation of P Elements in Drosophila melanogaster Can Be Elicited by Two P Copies at Cytological Site 1A on the X Chromosome
S. Ronsseray, M. Lehmann and D. Anxolabehere
Mecanismes Moleculaires de la Speciation, Universite Paris 6, 75005 Paris, France
Two P elements, inserted at the cytological site 1A on an X chromosome from an Drosophila melanogaster natural population (Lerik, USSR), were isolated by genetic methods to determine if they are sufficient to cause the P cytotype, the cellular condition that regulates the P family of transposable element. The resulting ``Lerik P(1A)'' line (abbreviated ``Lk-P(1A)'') carries only one P element in situ hybridization site but genomic Southern analysis indicates that this site contains two, probably full length, P copies separated by at least one EcoRI cleavage site. Because the Lk-P(1A) line shows some transposase activity, at least one of these two P elements is autonomous. The Lk-P(1A) line fully represses germline P element activity as judged by the GD sterility and sn(w) hypermutability assays; this result shows that the P cytotype can be elicited by only two P element copies. However, the Lk-P(1A) line does not fully repress {Delta}2-3(99B) transposase activity in the soma, although it fully represses {Delta}2-3(99B) transposase activity in the germline ({Delta}2-3(99B) is an in vitro modified P element that produces a high level of transposase activity in both the germline and the soma). The germline regulatory properties of the Lk-P(1A) line are maternally transmitted, even when the {Delta}2-3(99B) element is used as the source of transposase. By contrast, the partial regulation of {Delta}2-3(99B) somatic activity is chromosomally inherited. These results suggest that the regulatory P elements of the Lk-P(1A) line are inserted near a germline-specific enhancer.
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