Analysis of a Circular Derivative of Saccharomyces cerevisiae Chromosome III: A Physical Map and Identification and Location of ARS Elements

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Analysis of a Circular Derivative of Saccharomyces cerevisiae Chromosome III: A Physical Map and Identification and Location of ARS Elements

C. S. Newlon, L. R. Lipchitz, I. Collins, A. Deshpande, R. J. Devenish, R. P. Green, H. L. Klein, T. G. Palzkill, R. Ren, S. Synn and S. T. Woody
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, New Jersey 07103, Department of Biology, University of Iowa, Iowa City, Iowa 52242

DNA was isolated from a circular derivative of chromosome III to prepare a library of recombinant plasmids enriched in chromosome III sequences. An ordered set of recombinant plasmids and bacteriophages carrying the contiguous 210-kilobase region of chromosome III between the HML and MAT loci was identified, and a complete restriction map was prepared with BamHI and EcoRI. Using the high frequency transformation assay and extensive subcloning, 13 ARS elements were mapped in the cloned region. Comparison of the physical maps of chromosome III from three strains revealed that the chromosomes differ in the number and positions of Ty elements and also show restriction site polymorphisms. A comparison of the physical map with the genetic map shows that meiotic recombination rates vary at least tenfold along the length of chromosome.

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				K. Sharma, M. Weinberger, and J. A. Huberman Roles for Internal and Flanking Sequences in Regulating the Activity of Mating-Type-Silencer-Associated Replication Origins in Saccharomyces cerevisiae Genetics, September 1, 2001; 159(1): 35 - 45. [Abstract] [Full Text] [PDF]

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				J. F. Theis, C. Yang, C. B. Schaefer, and C. S. Newlon DNA Sequence and Functional Analysis of Homologous ARS Elements of Saccharomyces cerevisiae and S. carlsbergensis Genetics, July 1, 1999; 152(3): 943 - 952. [Abstract] [Full Text]

				S. M. Burgess, N. Kleckner, and a. B. M. Weiner Somatic pairing of homologs in budding yeast: existence and modulation Genes & Dev., June 15, 1999; 13(12): 1627 - 1641. [Abstract] [Full Text]

				Y. Li, T. Kane, C. Tipper, P. Spatrick, and D. D. Jenness Yeast Mutants Affecting Possible Quality Control of Plasma Membrane Proteins Mol. Cell. Biol., May 1, 1999; 19(5): 3588 - 3599. [Abstract] [Full Text] [PDF]

				C. Wu, K. Weiss, C. Yang, M. A. Harris, B.-K. Tye, C. S. Newlon, R. T. Simpson, and J. E. Haber Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching Genes & Dev., June 1, 1998; 12(11): 1726 - 1737. [Abstract] [Full Text]

				J. F. Theis and C. S. Newlon The ARS309 chromosomal replicator of Saccharomyces cerevisiae depends on an exceptional ARS consensus sequence PNAS, September 30, 1997; 94(20): 10786 - 10791. [Abstract] [Full Text] [PDF]

				K L Friedman, J D Diller, B M Ferguson, S V Nyland, B J Brewer, and W L Fangman Multiple determinants controlling activation of yeast replication origins late in S phase. Genes & Dev., July 1, 1996; 10(13): 1595 - 1607. [Abstract] [PDF]

				B. Brewer and W. Fangman Initiation at closely spaced replication origins in a yeast chromosome Science, December 10, 1993; 262(5140): 1728 - 1731. [Abstract] [PDF]