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Genetics, Vol 128, 663-671, Copyright © 1991
INVESTIGATIONS |
Second-Site Suppressors of a Cold-Sensitive Prohead Accessory Protein of Bacteriophage {phi}X174
B. A. Fane and M. Hayashi
Department of Biology, University of California at San Diego, La Jolla, California 92093
This study describes the isolation of second-site suppressors which correct for the defects associated with cold-sensitive (cs) prohead accessory proteins of bacteriophage {phi}X174. Five phenotypically different suppressors were isolated. Three of these suppressors confer novel temperature-sensitive (ts) phenotypes. They were unable to complement a ts mutation in gene F which encodes the major coat protein of the phage. All five suppressor mutations confer nucleotide changes in the gene F DNA sequence. These changes define four amino acid sites in the gene F protein. Three suppressor mutations placed into an otherwise wild-type background display a cold resistant phenotype in liquid culture infections when compared to a wild-type {phi}X174 control.
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