- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Zweifel, S. G.
- Articles by Fangman, W. L.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Zweifel, S. G.
- Articles by Fangman, W. L.
Genetics, Vol 128, 241-249, Copyright © 1991
INVESTIGATIONS |
A Nuclear Mutation Reversing a Biased Transmission of Yeast Mitochondrial DNA
S. G. Zweifel and W. L. Fangman
Department of Genetics, University of Washington, Seattle, Washington 98195
The highly biased transmission of {rho}(-) mitochondrial DNA that occurs in hypersuppressive matings between {rho}(-) and {rho}(+) cells of the yeast Saccharomyces cerevisiae is thought to be a consequence of the replication advantage of the {rho}(-) mtDNA. A nuclear gene, MGT1, that is required for this displacement of {rho}(+) mtDNA from zygotic clones has been identified through mutation. When one haploid parent carries the mgt1 allele, transmission of {rho}(-) mtDNA is substantially reduced. When both haploid parents carry the mgt1 allele, {rho}(-) mtDNA is essentially eliminated from the zygotic progeny. Thus in the absence of the MGT1 gene there is a switch in the transmission bias; {rho}(+) mtDNA rather than the hypersuppressive {rho}(-) mtDNA is inherited by most zygotic clones. In contrast to its semi-dominant behavior in haploid matings, mgt1 behaves as a recessive allele in diploid matings since the {rho}(+) genome in MGT1/mgt1 diploids is efficiently displaced when mated with a MGT1/mgt1 hypersuppressive {rho}(-) diploid strain. We find that {rho}(+) genomes can be comaintained along with hypersuppressive {rho}(-) mtDNA for extended periods in clonal lines derived from MGT1 X mgt1 matings. However, as expected from the recessive nature of the mgt1 mutation, these {rho}(+) genomes are eventually eliminated. Our work indicates that MGT1 plays a crucial role in the competition for inheritance between hypersuppressive {rho}(-) mtDNAs and the {rho}(+) mitochondrial genome. The MGT1 gene product may be a component of a mtDNA replication system that acts preferentially at the rep sequences found in hypersuppressive mtDNAs.
This article has been cited by other articles:
 |
|
 |
|
  F. Ling, A. Hori, and T. Shibata DNA Recombination-Initiation Plays a Role in the Extremely Biased Inheritance of Yeast [rho-] Mitochondrial DNA That Contains the Replication Origin ori5 Mol. Cell. Biol., February 1, 2007; 27(3): 1133 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Taylor, C. Zeyl, and E. Cooke Conflicting levels of selection in the accumulation of mitochondrial defects in Saccharomyces cerevisiae PNAS, March 19, 2002; 99(6): 3690 - 3694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tang, G. Manfredi, M. Hirano, and E. A. Schon Maintenance of Human Rearranged Mitochondrial DNAs in Long-Term Cultured Transmitochondrial Cell Lines Mol. Biol. Cell, July 1, 2000; 11(7): 2349 - 2358. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. Contamine and M. Picard Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast Microbiol. Mol. Biol. Rev., June 1, 2000; 64(2): 281 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sedman, S. Kuusk, S. Kivi, and J. Sedman A DNA Helicase Required for Maintenance of the Functional Mitochondrial Genome in Saccharomyces cerevisiae Mol. Cell. Biol., March 1, 2000; 20(5): 1816 - 1824. [Abstract] [Full Text]
|
|
|
|
 |
|
 O. Zelenaya-Troitskaya, S. M. Newman, K. Okamoto, P. S. Perlman, and R. A. Butow Functions of the High Mobility Group Protein, Abf2p, in Mitochondrial DNA Segregation, Recombination and Copy Number in Saccharomyces cerevisiae Genetics, April 1, 1998; 148(4): 1763 - 1776. [Abstract] [Full Text] [PDF]
|
|