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Genetics, Vol 127, 601-607, Copyright © 1991
INVESTIGATIONS |
Photoregulation of the Cat2 and Cat3 Catalase Genes in Pigmented and Pigment-Deficient Maize: The Circadian Regulation of Cat3 Is Superimposed on Its Quasi-Constitutive Expression in Maize Leaves
A. Acevedo, J. D. Williamson and J. G. Scandalios
Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695-7614
We have investigated the accumulation of Cat2 and Cat3 catalase transcripts in 6-7-day postimbibition leaves of normally pigmented and pigment-deficient maize seedlings under different light regimes. In seedlings of normal inbred maize lines Cat2 mRNA accumulates to significantly higher levels in either continuous light or a diurnal light/dark cycle than in continuous dark. In contrast to the high levels of the Cat2 message observed in their wild-type siblings, carotenoid-deficient mutants accumulate Cat2 mRNA at barely detectable levels. Mutants deficient in chlorophylls, but having normal carotenoid levels, accumulate normal levels of Cat2 mRNA. This suggests that both light and carotenoids are required for the normal accumulation of the Cat2 message. The steady-state level of Cat3 RNA exhibits a dramatic diurnal variation when seedlings are grown under a 24-hr light/dark cycle. We have previously shown that this variation is at the level of Cat3 gene transcription and is under the control of a novel circadian rhythm. In this study we show that both pigment-deficient mutants and their wild-type siblings exhibit the normal diurnal pattern of Cat3 RNA accumulation. This indicates that photosynthetic pigments, allelic variation, and genetic background do not directly affect the temporal pattern of Cat3 accumulation in leaves. We observed, however, that when normal plants are grown in either continuous light or continuous dark, the Cat3 transcript in leaves is present at uniformly high levels throughout the 24-hr sampling period. Because the Cat3 gene is continually transcribed in leaves in the absence of a cyclic light regime, the normally observed diurnal variation of Cat3 gene expression is apparently the result of a circadian-regulated transcriptional repressor.
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