- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Gray, M.
- Articles by Bender, W.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Gray, M.
- Articles by Bender, W.
Genetics, Vol 127, 139-149, Copyright © 1991
INVESTIGATIONS |
Mapping Point Mutations in the Drosophila rosy Locus Using Denaturing Gradient Gel Blots
M. Gray, A. Charpentier, K. Walsh, P. Wu and W. Bender
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, and Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Tufts University School of Medicine, Boston, Massachusetts 02111
Mutations within the rosy locus of Drosophila were mapped using blots of genomic DNA fragments separated on denaturing gradient gels. DNA sequence differences between otherwise identical small rosy DNA fragments were detected among the mutants as mobility shifts on the blots. Mutations were mapped to within a few hundred base pairs of rosy sequence in 100 of 130 mutants tested--a 77% detection rate. The sequence changes in 43 rosy mutations are presented; all but six of these were single base changes. Thirty-four of 36 sequenced mutations induced by the alkylating agents N-ethyl-N-nitrosourea and ethyl methanesulfonate were transitions. All of the mutations mapped in the rosy transcription unit. Twenty-three of the 43 sequenced mutations change the predicted rosy gene polypeptide sequence; the remainder would interrupt protein translation (17), or disrupt mRNA processing (3).
This article has been cited by other articles:
 |
|
 |
|
  K. Beumer, G. Bhattacharyya, M. Bibikova, J. K. Trautman, and D. Carroll Efficient Gene Targeting in Drosophila With Zinc-Finger Nucleases Genetics, April 1, 2006; 172(4): 2391 - 2403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Myster, F. Wang, R. Cavallo, W. Christian, S. Bhotika, C. T. Anderson, and M. Peifer Genetic and Bioinformatic Analysis of 41C and the 2R Heterochromatin of Drosophila melanogaster: A Window on the Heterochromatin-Euchromatin Junction Genetics, February 1, 2004; 166(2): 807 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Calvi, M. A. Lilly, and A. C. Spradling Cell cycle control of chorion gene amplification Genes & Dev., March 1, 1998; 12(5): 734 - 744. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Glatigny and C. Scazzocchio Cloning and Molecular Characterization of hxA, the Gene Coding for the Xanthine Dehydrogenase (Purine Hydroxylase I) of Aspergillus nidulans J. Biol. Chem., February 24, 1995; 270(8): 3534 - 3550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R G Ramos, G L Igloi, B Lichte, U Baumann, D Maier, T Schneider, J H Brandstatter, A Frohlich, and K F Fischbach The irregular chiasm C-roughest locus of Drosophila, which affects axonal projections and programmed cell death, encodes a novel immunoglobulin-like protein. Genes & Dev., December 1, 1993; 7(12b): 2533 - 2547. [Abstract] [PDF]
|
|
|
|
|
|
S J Bray and F C Kafatos Developmental function of Elf-1: an essential transcription factor during embryogenesis in Drosophila. Genes & Dev., September 1, 1991; 5(9): 1672 - 1683. [Abstract] [PDF]
|
|