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Genetics, Vol 126, 949-960, Copyright © 1990
INVESTIGATIONS |
P Element Regulatory Products Enhance zeste(1) Repression of a P[white(duplicated)] Transgene in Drosophila melanogaster
D. Coen
Mecanismes Moleculaires de la Speciation, Universite Pierre et Marie Curie, F-75005 Paris, France
Drosophila P element mobilization is subject to a complex array of regulatory mechanisms. A fruitful approach to study them is the use of insertion mutations whose expression is influenced by P regulation. In the present report, it is shown that P element somatic products may influence the expression of an unrelated gene inserted in a P transposon. The P[w(d1)9.3]19DE transgene carries an in vitro modified white gene harboring a duplication of the 5' regulatory sequences. Expression of this transgene is repressed in a P background. No maternal effect is detected and repression can be relieved as soon as P chromosomes are replaced by M ones. The amplitude of repression is correlated to the P transposase activity of the individuals examined. Repression appears to be exerted by somatic products of complete autonomous P elements or of in vitro modified P elements lacking the capacity to express the fourth P exon. The P repression of P[w(d1)9.3]19DE is strongly dependent on the insertion site of this transgene. This P repression effect occurs only in the presence of the zeste(1) allele and is suppressed by Su(z)2 mutations. No qualitative differences of transcription pattern are observed between white(+) and P[w(d1)9.3]19DE in any backgrounds. P repression acts to reduce the amount of the major white transcript. This suggests that P regulatory products may act through cis-interactions at a distance of over 3 kb.
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  S. E. Roche and D. C. Rio Trans-Silencing by P Elements Inserted in Subtelomeric Heterochromatin Involves the Drosophila Polycomb Group Gene, Enhancer of zeste Genetics, August 1, 1998; 149(4): 1839 - 1855. [Abstract] [Full Text] [PDF]
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 S E Roche, M Schiff, and D C Rio P-element repressor autoregulation involves germ-line transcriptional repression and reduction of third intron splicing. Genes & Dev., May 15, 1995; 9(10): 1278 - 1288. [Abstract] [PDF]
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