- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Voelkel-Meiman, K.
- Articles by Roeder, G. S.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Voelkel-Meiman, K.
- Articles by Roeder, G. S.
Genetics, Vol 126, 851-867, Copyright © 1990
INVESTIGATIONS |
Gene Conversion Tracts Stimulated by HOT1-Promoted Transcription Are Long and Continuous
K. Voelkel-Meiman and G. S. Roeder
Department of Biology, Yale University, New Haven, Connecticut 06511-8112
The recombination-stimulating sequence, HOT1, corresponds to the promoter of transcription by yeast RNA polymerase I. The effect of HOT1 on mitotic interchromosomal recombination was examined in diploid strains carrying a heterozygous URA3 gene on chromosome III. The frequency of Ura(-) recombinants was increased 20-fold when HOT1 was inserted into the chromosome III copy marked with URA3, at a location 48 kbp centromere-proximal to URA3. Ura(-) recombinants were increased only 2-fold when HOT1 and URA3 were on opposite homologues. These results suggest that most HOT1-promoted Ura(-) recombinants result from gene conversion and that sequences on the HOT1-containing chromosome are preferentially converted. Characterization of Ura(-) recombinants isolated from strains carrying multiple markers on chromosome III indicates that HOT1-promoted gene conversion tracts are unusually long (often>75 kbp) and almost always continuous. Furthermore, conversion tracts frequently extend to both sides of HOT1. We suggest that HOT1 promotes the formation of a double-strand break which is often followed by exonucleolytic digestion. Repair of the broken chromosome could then result from gap repair or from replicative repair primed only by the centromere-containing chromosomal fragment.
This article has been cited by other articles:
 |
|
 |
|
  A. Deem, K. Barker, K. VanHulle, B. Downing, A. Vayl, and A. Malkova Defective Break-Induced Replication Leads to Half-Crossovers in Saccharomyces cerevisiae Genetics, August 1, 2008; 179(4): 1845 - 1860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Schildkraut, C. A. Miller, and J. A. Nickoloff Transcription of a Donor Enhances Its Use during Double-Strand Break-Induced Gene Conversion in Human Cells Mol. Cell. Biol., April 15, 2006; 26(8): 3098 - 3105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Vinogradov Isochores and tissue-specificity Nucleic Acids Res., September 1, 2003; 31(17): 5212 - 5220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Achaz, P. Netter, and E. Coissac Study of Intrachromosomal Duplications Among the Eukaryote Genomes Mol. Biol. Evol., December 1, 2001; 18(12): 2280 - 2288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Cox Historical overview: Searching for replication help in all of the rec places PNAS, July 17, 2001; 98(15): 8173 - 8180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kraus, W.-Y. Leung, and J. E. Haber Break-induced replication: A review and an example in budding yeast PNAS, July 17, 2001; 98(15): 8255 - 8262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Malagon and A. Aguilera Yeast spt6-140 Mutation, Affecting Chromatin and Transcription, Preferentially Increases Recombination in Which Rad51p-Mediated Strand Exchange Is Dispensable Genetics, June 1, 2001; 158(2): 597 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Malkova, L. Signon, C. B. Schaefer, M. L. Naylor, J. F. Theis, C. S. Newlon, and J. E. Haber RAD51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site Genes & Dev., May 1, 2001; 15(9): 1055 - 1060. [Abstract] [Full Text]
|
|
|
|
|
|
L. Signon, A. Malkova, M. L. Naylor, H. Klein, and J. E. Haber Genetic Requirements for RAD51- and RAD54-Independent Break-Induced Replication Repair of a Chromosomal Double-Strand Break Mol. Cell. Biol., March 15, 2001; 21(6): 2048 - 2056. [Abstract] [Full Text]
|
|
|
|
|
|
A. Malkova, F. Klein, W.-Y. Leung, and J. E. Haber HO endonuclease-induced recombination in yeast meiosis resembles Spo11-induced events PNAS, December 19, 2000; 97(26): 14500 - 14505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Saxe, A. Datta, and S. Jinks-Robertson Stimulation of Mitotic Recombination Events by High Levels of RNA Polymerase II Transcription in Yeast Mol. Cell. Biol., August 1, 2000; 20(15): 5404 - 5414. [Abstract] [Full Text]
|
|
|
|
|
|
L. Li, C. A. Peterson, X. Zhang, and R. J. Legerski Requirement for PCNA and RPA in interstrand crosslink-induced DNA synthesis Nucleic Acids Res., March 15, 2000; 28(6): 1424 - 1427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Nickoloff, D. B. Sweetser, J. A. Clikeman, G. J. Khalsa, and S. L. Wheeler Multiple Heterologies Increase Mitotic Double-Strand Break-Induced Allelic Gene Conversion Tract Lengths in Yeast Genetics, October 1, 1999; 153(2): 665 - 679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Paques and J. E. Haber Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 349 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kuzminov and F. W. Stahl Double-strand end repair via the RecBC pathway in Escherichia coli primes DNA replication Genes & Dev., February 1, 1999; 13(3): 345 - 356. [Abstract] [Full Text]
|
|