Genetic Analysis of the Enhancer of zeste Locus and Its Role in Gene Regulation in Drosophila melanogaster

INVESTIGATIONS

Genetic Analysis of the Enhancer of zeste Locus and Its Role in Gene Regulation in Drosophila melanogaster

R. S. Jones and W. M. Gelbart
Current address: Department of Biological Sciences, Fondren Science Building, Southern Methodist University, Dallas, Texas 75275-0376.

The Enhancer of zeste [E(z)] locus of Drosophila melanogaster is implicated in multiple examples of gene regulation during development. First identified as dominant gain-of-function modifiers of the zeste(1)-white (z-w) interaction, mutant E(z) alleles also produce homeotic transformations. Reduction of E(z)(+) activity leads to both suppression of the z-w interaction and ectopic expression of segment identity genes of the Antennapedia and bithorax gene complexes. This latter effect defines E(z) as a member of the Polycomb-group of genes. Analysis of E(z)(s2), a temperature-sensitive E(z) allele, reveals that both maternally and zygotically produced E(z)(+) activity is required to correctly regulate the segment identity genes during embryonic and imaginal development. As has been shown for other Polycomb-group genes, E(z)(+) is required not to initiate the pattern of these genes, but rather to maintain their repressed state. We propose that the E(z) loss-of-function eye color and homeotic phenotypes may both be due to gene derepression, and that the E(z)(+) product may be a general repressing factor required for both examples of negative gene regulation.

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				L. Wang, N. Jahren, M. L. Vargas, E. F. Andersen, J. Benes, J. Zhang, E. L. Miller, R. S. Jones, and J. A. Simon Alternative ESC and ESC-Like Subunits of a Polycomb Group Histone Methyltransferase Complex Are Differentially Deployed during Drosophila Development. Mol. Cell. Biol., April 1, 2006; 26(7): 2637 - 2647. [Abstract] [Full Text] [PDF]

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