- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Daegelen, P.
- Articles by Brody, E.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Daegelen, P.
- Articles by Brody, E.
Genetics, Vol 125, 237-248, Copyright © 1990
INVESTIGATIONS |
The rIIA Gene of Bacteriophage T4. I. Its DNA Sequence and Discovery of a New Open Reading Frame Between Genes 60 and rIIA
P. Daegelen and E. Brody
Present address: Groupe Systemes Paralleles et Biologie, Institut de Biotechnologie, I.N.R.A. Domaine de Vilvert, 78350 Jouy en Josas, France.
We have determined the DNA sequence of the rIIA gene and have discovered a small open reading frame, rIIA.1, between genes 60 and rIIA. The predicted molecular weights of these proteins are 82,840 for rIIA and 8,124 for rIIA.1. The rIIA protein has a repeated motif which suggests that the gene has evolved by duplication. It also has a motif which suggests that it belongs to a group of ompR-like proteins that control regulation of gene expression in response to changes in the external environment. We have sequenced three different missense mutants whose mutations lie in the Ala segment of the rIIA genetic map. All three changes are found within the first 35 bp of the rIIA coding sequence. The region of control of protein synthesis is identical in the rIIA gene and in gene 44 of T4. We relate this finding to the high sensitivity of both RNAs to translational repression by the T4 regA gene product.