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Genetics, Vol 125, 21-27, Copyright © 1990
INVESTIGATIONS |
GAL4 Mutations That Separate the Transcriptional Activation and GAL80-Interactive Functions of the Yeast GAL4 Protein
J. M. Salmeron-Jr., K. K. Leuther and S. A. Johnston
Department of Botany, Duke University, Durham, North Carolina 27706
The carboxy-terminal 28 amino acids of the Saccharomyces cerevisiae transcriptional activator protein GAL4 execute two functions--transcriptional activation and interaction with the negative regulatory protein, GAL80. Here we demonstrate that these two functions are separable by single amino acid changes within this region. We determined the sequences of four GAL4(C)-mutations, and characterized the abilities of the encoded GAL4(C) proteins to activate transcription of the galactose/melibiose regulon in the presence of GAL80 and superrepressible GAL80(S) alleles. One of the GAL4(C) mutations can be compensated by a specific GAL80(S) mutation, resulting in a wild-type phenotype. These results support the idea that while the GAL4 activation function tolerates at least minor alterations in the GAL4 carboxyl terminus, the GAL80-interactive function is highly sequence-specific and sensitive even to single amino acid alterations. They also argue that the GAL80(S) mutations affect the affinity of GAL80 for GAL4, and not the ability of GAL80 to bind inducer.
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