Genetics, Vol 125, 115-127, Copyright © 1990

INVESTIGATIONS

The Genetic Analysis of Distributive Segregation in Drosophila melanogaster. II. Further Genetic Analysis of the nod Locus

P. Zhang and R. S. Hawley
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461

In Drosophila melanogster females the segregation of nonexchange chromosomes is ensured by the distributive segregation system. The mutation nod(a) specifically impairs distributive disjunction and induces nonexhcange chromosomes to undergo nondisjunction, as well as both meiotic and mitotic chromosome loss. We report here the isolation of seven recessive X-linked mutations that are allelic to nod(a). As homozygotes, all of these mutations exhibit a phenotype that is similar to that exhibited by nod(a) homozygotes. We have also used these mutations to demonstrate that nod mutations induce nonexchange chromosomes to nondisjoin at meiosis II. Our data demonstrate that the effects of nod(a) on meiotic chromosome behavior are a general property of mutations at the nod locus. Several of these mutations exhibit identical phenotypes as homozygotes and as heterozygotes with a deficiency for the nod locus; these likely correspond to complete loss-of-function or null alleles. None of these mutations causes lethality, decreases the frequency of exchange, or impairs the disjunction of exchange chromosomes in females. Thus, either the nod locus defines a function that is specific to distributive segregation or exchange can fully compensate for the absence of the nod(+) function.

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