- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Petitjean, A.
- Articles by Tatchell, K.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Petitjean, A.
- Articles by Tatchell, K.
Genetics, Vol 124, 797-806, Copyright © 1990
INVESTIGATIONS |
Comparison of Thermosensitive Alleles of the CDC25 Gene Involved in the cAMP Metabolism of Saccharomyces cerevisiae
A. Petitjean, F. Hilger and K. Tatchell
Faculte des Sciences Agronomiques, Laboratoire de Microbiologie, 6 Avenue Marechal Juin, 5800 Gambloux, Belgium
The CDC25 gene from Saccharomyces cerevisiae is an essential component of the RAS-adenylate cyclase pathway. Genetic and biochemical evidence has led to the proposal that the gene product may act upstream of RAS, possibly as a guanine nucleotide exchange factor. We report here the cloning, sequencing and characterization of four mutations in the CDC25 gene. All four are missense mutations which reside within the carboxy-terminal quarter of the single open reading frame found within the gene. Three of the four are missense mutations in the same amino acid codon. A search of protein data bases reveals that the carboxy terminus of the putative CDC25 gene product is similar to that of LTE1, a gene required for growth at low temperature and SCD25, a suppressor of cdc25. Taken together these data indicate that the carboxy terminus of CDC25 plays a critical role in the function of the CDC25 gene product and that other proteins, such as LTE1 or SCD25, may have related activities.
This article has been cited by other articles:
 |
|
 |
|
  J. L. Folch-Mallol, L. M. Martinez, S. J. Casas, R. Yang, C. Martinez-Anaya, L. Lopez, A. Hernandez, and J. Nieto-Sotelo New roles for CDC25 in growth control, galactose regulation and cellular differentiation in Saccharomyces cerevisiae Microbiology, September 1, 2004; 150(9): 2865 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Kohler and K. M. Muller Adaptation of the Ras-recruitment system to the analysis of interactions between membrane-associated proteins Nucleic Acids Res., March 15, 2003; 31(6): e28 - e28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kohler A yeast-based growth assay for the analysis of site-specific proteases Nucleic Acids Res., February 15, 2003; 31(4): e16 - e16. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Andreasson and P. O. Ljungdahl Receptor-mediated endoproteolytic activation of two transcription factors in yeast Genes & Dev., December 15, 2002; 16(24): 3158 - 3172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chomchan, S.-F. Li, and Y. Shirako Rice Grassy Stunt Tenuivirus Nonstructural Protein p5 Interacts with Itself To Form Oligomeric Complexes In Vitro and In Vivo J. Virol., December 6, 2002; 77(1): 769 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hendron, P. Patel, M. Hausenfluke, N. Gamper, M. S. Shapiro, R. E. Booth, and J. D. Stockand Identification of Cytoplasmic Domains within the Epithelial Na+ Channel Reactive at the Plasma Membrane J. Biol. Chem., September 6, 2002; 277(37): 34480 - 34488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Poirey, L. Despons, V. Leh, M.-J. Lafuente, S. Potier, J.-L. Souciet, and J.-C. Jauniaux Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability Microbiology, July 1, 2002; 148(7): 2111 - 2123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hubsman, G. Yudkovsky, and A. Aronheim A novel approach for the identification of protein-protein interaction with integral membrane proteins Nucleic Acids Res., February 15, 2001; 29(4): e18 - e18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Forsberg and P. O. Ljungdahl Genetic and Biochemical Analysis of the Yeast Plasma Membrane Ssy1p-Ptr3p-Ssy5p Sensor of Extracellular Amino Acids Mol. Cell. Biol., February 1, 2001; 21(3): 814 - 826. [Abstract] [Full Text]
|
|
|
|
|
|
R. Yang, K. T. Chun, and R. C. Wek Mitochondrial Respiratory Mutants in Yeast Inhibit Glycogen Accumulation by Blocking Activation of Glycogen Synthase J. Biol. Chem., November 20, 1998; 273(47): 31337 - 31344. [Abstract] [Full Text] [PDF]
|
|