- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Wakem, L. P.
- Articles by Sherman, F.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Wakem, L. P.
- Articles by Sherman, F.
Genetics, Vol 124, 515-522, Copyright © 1990
INVESTIGATIONS |
Isolation and Characterization of Omnipotent Suppressors in the Yeast Saccharomyces cerevisiae
L. P. Wakem and F. Sherman
Departments of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642
Approximately 290 omnipotent suppressors, which enhance translational misreading, were isolated in strains of the yeast Saccharomyces cerevisiae containing the {psi}(+) extrachromosomal determinant. The suppressors could be assigned to 8 classes by their pattern of suppression of five nutritional markers. The suppressors were further distinguished by differences in growth on paromomycin medium, hypertonic medium, low temperatures (10{deg}), nonfermentable carbon sources, {alpha}-aminoadipic acid medium, and by their dominance and recessiveness. Genetic analysis of 12 representative suppressors resulted in the assignment of these suppressors to 6 different loci, including the three previously described loci SUP35 (chromosome IV), SUP45 (chromosome II) and SUP46 (chromosome II), as well as three new loci SUP42 (chromosome IV), SUP43 (chromosome XV) and SUP44 (chromosome VII). Suppressors belonging to the same locus had a wide range of different phenotypes. Differences between alleles of the same locus and similarities between alleles of different loci suggest that the omnipotent suppressors encode proteins that effect different functions and that altered forms of each of the proteins can effect the same function.
This article has been cited by other articles:
 |
|
 |
|
  M. Rakwalska and S. Rospert The Ribosome-Bound Chaperones RAC and Ssb1/2p Are Required for Accurate Translation in Saccharomyces cerevisiae Mol. Cell. Biol., October 15, 2004; 24(20): 9186 - 9197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Namy, I. Hatin, G. Stahl, H. Liu, S. Barnay, L. Bidou, and J.-P. Rousset Gene Overexpression as a Tool for Identifying New trans-Acting Factors Involved in Translation Termination in Saccharomyces cerevisiae Genetics, June 1, 2002; 161(2): 585 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chacinska, M. Boguta, J. Krzewska, and S. Rospert Prion-Dependent Switching between Respiratory Competence and Deficiency in the Yeast nam9-1 Mutant Mol. Cell. Biol., October 1, 2000; 20(19): 7220 - 7229. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. R. Greenberg, L. Phan, Z. Gu, A. deSilva, C. Apolito, F. Sherman, A. G. Hinnebusch, and D. S. Goldfarb Nip1p Associates with 40 S Ribosomes and the Prt1p Subunit of Eukaryotic Initiation Factor 3 and Is Required for Efficient Translation Initiation J. Biol. Chem., September 4, 1998; 273(36): 23485 - 23494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chernoff, S. Lindquist, B Ono, S. Inge-Vechtomov, and S. Liebman Role of the chaperone protein Hsp104 in propagation of the yeast prion-like factor [psi+] Science, May 12, 1995; 268(5212): 880 - 884. [Abstract] [PDF]
|
|