- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Lemmon, S. K.
- Articles by Jones, E. W.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Lemmon, S. K.
- Articles by Jones, E. W.
Genetics, Vol 124, 27-38, Copyright © 1990
INVESTIGATIONS |
Genetic Instability of Clathrin-Deficient Strains of Saccharomyces cerevisiae
S. K. Lemmon, C. Freund, K. Conley and E. W. Jones
Current address: Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Saccharomyces cerevisiae strains carrying a mutation in the clathrin heavy chain gene (CHC1) are genetically unstable and give rise to heterogeneous populations of cells. Manifestations of the instability include increases in genome copy number as well as compensatory genetic changes that allow better growing clathrin-deficient cells to take over the population. Increases in genome copy number appear to result from changes in ploidy as well as alterations in normal nuclear number. Genetic background influences the frequency at which cells with increased genome content are observed in different Chc(-) strains. We cannot distinguish whether genetic background affects the rate at which aberrant nuclear division events occur or a growth advantage of cells with increased nuclear and/or genome content. However, survival of chc1-{Delta} cells does not require an increase in genome copy number. The clathrin heavy chain gene was mapped 1-2 cM distal to KEX1 on the left arm of chromosome VII by making use of integrated 2{mu} plasmid sequences to destabilize distal chromosome segments and allow ordering of the genes.
This article has been cited by other articles:
 |
|
 |
|
  Z. Hua, P. Fatheddin, and T. R. Graham An Essential Subfamily of Drs2p-related P-Type ATPases Is Required for Protein Trafficking between Golgi Complex and Endosomal/Vacuolar System Mol. Biol. Cell, September 1, 2002; 13(9): 3162 - 3177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Henry, K. D'Hondt, J. Chang, T. Newpher, K. Huang, R. T. Hudson, H. Riezman, and S. K. Lemmon Scd5p and Clathrin Function Are Important for Cortical Actin Organization, Endocytosis, and Localization of Sla2p in Yeast Mol. Biol. Cell, August 1, 2002; 13(8): 2607 - 2625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Chen, M. F. Ingram, P. H. Rosal, and T. R. Graham Role for Drs2p, a P-Type ATPase and Potential Aminophospholipid Translocase, in Yeast Late Golgi Function J. Cell Biol., December 13, 1999; 147(6): 1223 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Chen and T. R. Graham An arf1{Delta} Synthetic Lethal Screen Identifies a New Clathrin Heavy Chain Conditional Allele That Perturbs Vacuolar Protein Transport in Saccharomyces cerevisiae Genetics, October 1, 1998; 150(2): 577 - 589. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Huang, L Gullberg, K. Nelson, C. Stefan, K Blumer, and S. Lemmon Novel functions of clathrin light chains: clathrin heavy chain trimerization is defective in light chain-deficient yeast J. Cell Sci., January 4, 1997; 110(7): 899 - 910. [Abstract] [PDF]
|
|