Modified P Elements That Mimic the P Cytotype in Drosophila melanogaster

INVESTIGATIONS

Modified P Elements That Mimic the P Cytotype in Drosophila melanogaster

H. M. Robertson and W. R. Engels
Department of Entomology, University of Illinois, Urbana, Illinois 61801

Activity of the P family of transposable elements in Drosophila melanogaster is regulated primarily by a cellular condition known as P cytotype. It has been hypothesized that P cytotype depends on a P element-encoded repressor of transposition and excision. We provide evidence in support of this idea by showing that two modified P elements, each with lesions affecting the fourth transposase exon, mimic most of the P cytotype effects. These elements were identified by means of two sensitive assays capable of detecting repression by a single P element. One assay makes use of cytotype-dependent gene expression of certain P element insertion mutations at the singed bristle locus. The other measures suppression of transposase activity from the unusually stable genomic P element, {Delta}2-3(99B), that normally produces transposase in both germinal and somatic tissues. The P cytotype-like effects include suppression of sn(w) germline hypermutability, sn(w) somatic mosaicism, pupal lethality, and gonadal dysgenic sterility. Unlike P cytotype, however, there was no reciprocal cross effect in the inheritance of repression.

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				L. Marin, M. Lehmann, D. Nouaud, H. Izaabel, D. Anxolabéhère, and S. Ronsseray P-Element Repression in Drosophila melanogaster by a Naturally Occurring Defective Telomeric P Copy Genetics, August 1, 2000; 155(4): 1841 - 1854. [Abstract] [Full Text]

				D. Lisch, L. Girard, M. Donlin, and M. Freeling Functional Analysis of Deletion Derivatives of the Maize Transposon MuDR Delineates Roles for the MURA and MURB Proteins Genetics, January 1, 1999; 151(1): 331 - 341. [Abstract] [Full Text]

				S. E. Roche and D. C. Rio Trans-Silencing by P Elements Inserted in Subtelomeric Heterochromatin Involves the Drosophila Polycomb Group Gene, Enhancer of zeste Genetics, August 1, 1998; 149(4): 1839 - 1855. [Abstract] [Full Text] [PDF]

				S. Ronsseray, L. Marin, M. Lehmann, and D. Anxolabehere Repression of Hybrid Dysgenesis in Drosophila melanogaster by Combinations of Telomeric P-Element Reporters and Naturally Occurring P Elements Genetics, August 1, 1998; 149(4): 1857 - 1866. [Abstract] [Full Text] [PDF]

				M. G. Kidwell and D. Lisch Transposable elements as sources of variation in animals and plants PNAS, July 22, 1997; 94(15): 7704 - 7711. [Abstract] [Full Text] [PDF]

				J Mihaly, I Hogga, J Gausz, H Gyurkovics, and F Karch In situ dissection of the Fab-7 region of the bithorax complex into a chromatin domain boundary and a Polycomb-response element Development, January 5, 1997; 124(9): 1809 - 1820. [Abstract] [PDF]

				S E Roche, M Schiff, and D C Rio P-element repressor autoregulation involves germ-line transcriptional repression and reduction of third intron splicing. Genes & Dev., May 15, 1995; 9(10): 1278 - 1288. [Abstract] [PDF]

				C W Siebel, A Admon, and D C Rio Soma-specific expression and cloning of PSI, a negative regulator of P element pre-mRNA splicing. Genes & Dev., February 1, 1995; 9(3): 269 - 283. [Abstract] [PDF]

				C W Siebel, L D Fresco, and D C Rio The mechanism of somatic inhibition of Drosophila P-element pre-mRNA splicing: multiprotein complexes at an exon pseudo-5' splice site control U1 snRNP binding. Genes & Dev., August 1, 1992; 6(8): 1386 - 1401. [Abstract] [PDF]

				T Maniatis Mechanisms of alternative pre-mRNA splicing Science, January 4, 1991; 251(4989): 33 - 34. [PDF]

				C. Siebel and D. Rio Regulated splicing of the Drosophila P transposable element third intron in vitro: somatic repression Science, June 8, 1990; 248(4960): 1200 - 1208. [Abstract] [PDF]