Meiotic Recombination-Deficient Mutants of Schizosaccharomyces pombe

INVESTIGATIONS

Meiotic Recombination-Deficient Mutants of Schizosaccharomyces pombe

A. S. Ponticelli and G. R. Smith
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, and Department of Pathology, University of Washington, Seattle, Washington 98155 Present address: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.

A mutant screen employing the ade6-M26 recombination hotspot was developed and used to isolate Schizosaccharomyces pombe mutants deficient in meiotic recombination. Nine rec mutations were recessive, defining six complementation groups, and reduced ade6 meiotic recombination 3-fold to >/=300-fold when homozygous. Three recessive rec mutations analyzed further also reduced meiotic intragenic recombination at ura4 on chromosome III and intergenic recombination between pro2 and arg3 on chromosome I. The observed non-co-ordinate reductions of the recombinant frequencies in the three test intervals suggest a degree of locus (or intragenic vs. intergenic) specificity of the corresponding rec(+) gene products. None of the mutations specifically inactivated the ade6-M26 hotspot. Additional rec genes may be identified with these methods.

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				L. Davis and G. R. Smith Dynein Promotes Achiasmate Segregation in Schizosaccharomyces pombe Genetics, June 1, 2005; 170(2): 581 - 590. [Abstract] [Full Text] [PDF]

				D. W. Pryce, A. Lorenz, J. B. Smirnova, J. Loidl, and R. J. McFarlane Differential Activation of M26-Containing Meiotic Recombination Hot Spots in Schizosaccharomyces pombe Genetics, May 1, 2005; 170(1): 95 - 106. [Abstract] [Full Text] [PDF]

				W. W. Steiner and G. R. Smith Optimizing the Nucleotide Sequence of a Meiotic Recombination Hotspot in Schizosaccharomyces pombe Genetics, April 1, 2005; 169(4): 1973 - 1983. [Abstract] [Full Text] [PDF]

				J. A. Farah, G. Cromie, W. W. Steiner, and G. R. Smith A Novel Recombination Pathway Initiated by the Mre11/Rad50/Nbs1 Complex Eliminates Palindromes During Meiosis in Schizosaccharomyces pombe Genetics, March 1, 2005; 169(3): 1261 - 1274. [Abstract] [Full Text] [PDF]

				G. A. Cromie, C. A. Rubio, R. W. Hyppa, and G. R. Smith A Natural Meiotic DNA Break Site in Schizosaccharomyces pombe Is a Hotspot of Gene Conversion, Highly Associated With Crossing Over Genetics, February 1, 2005; 169(2): 595 - 605. [Abstract] [Full Text] [PDF]

				C. Ellermeier, H. Schmidt, and G. R. Smith Swi5 Acts in Meiotic DNA Joint Molecule Formation in Schizosaccharomyces pombe Genetics, December 1, 2004; 168(4): 1891 - 1898. [Abstract] [Full Text] [PDF]

				M. K. Davidson, N. P. Young, G. G. Glick, and W. P. Wahls Meiotic chromosome segregation mutants identified by insertional mutagenesis of fission yeast Schizosaccharomyces pombe; tandem-repeat, single-site integrations Nucleic Acids Res., August 17, 2004; 32(14): 4400 - 4410. [Abstract] [Full Text] [PDF]

				J. A. Young, R. W. Hyppa, and G. R. Smith Conserved and Nonconserved Proteins for Meiotic DNA Breakage and Repair in Yeasts Genetics, June 1, 2004; 167(2): 593 - 605. [Abstract] [Full Text] [PDF]

				M. Molnar, E. Doll, A. Yamamoto, Y. Hiraoka, and J. Kohli Linear element formation and their role in meiotic sister chromatid cohesion and chromosome pairing J. Cell Sci., May 1, 2003; 116(9): 1719 - 1731. [Abstract] [Full Text] [PDF]

				L. Davis and G. R. Smith Nonrandom Homolog Segregation at Meiosis I in Schizosaccharomyces pombe Mutants Lacking Recombination Genetics, March 1, 2003; 163(3): 857 - 874. [Abstract] [Full Text] [PDF]

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				L. Davis and G. R. Smith Meiotic recombination and chromosome segregation in Schizosaccharomyces pombe PNAS, July 17, 2001; 98(15): 8395 - 8402. [Abstract] [Full Text] [PDF]

				M. Molnar, S. Parisi, Y. Kakihara, H. Nojima, A. Yamamoto, Y. Hiraoka, A. Bozsik, M. Sipiczki, and J. Kohli Characterization of rec7, an Early Meiotic Recombination Gene in Schizosaccharomyces pombe Genetics, February 1, 2001; 157(2): 519 - 532. [Abstract] [Full Text]

				M. Molnar, J. Bahler, J. Kohli, and Y. Hiraoka Live observation of fission yeast meiosis in recombination-deficient mutants: a study on achiasmate chromosome segregation J. Cell Sci., January 8, 2001; 114(15): 2843 - 2853. [Abstract] [Full Text] [PDF]

				M. D. Krawchuk and W. P. Wahls Centromere Mapping Functions for Aneuploid Meiotic Products: Analysis of rec8, rec10 and rec11 Mutants of the Fission Yeast Schizosaccharomyces pombe Genetics, September 1, 1999; 153(1): 49 - 55. [Abstract] [Full Text]

				M. D. Krawchuk, L. C. DeVeaux, and W. P. Wahls Meiotic Chromosome Dynamics Dependent Upon the rec8+, rec10+ and rec11+ Genes of the Fission Yeast Schizosaccharomyces pombe Genetics, September 1, 1999; 153(1): 57 - 68. [Abstract] [Full Text]

				A. Yamamoto, R. R. West, J. R. McIntosh, and Y. Hiraoka A Cytoplasmic Dynein Heavy Chain Is Required for Oscillatory Nuclear Movement of Meiotic Prophase and Efficient Meiotic Recombination in Fission Yeast J. Cell Biol., June 14, 1999; 145(6): 1233 - 1250. [Abstract] [Full Text] [PDF]

				S. Parisi, M. J. McKay, M. Molnar, M. A. Thompson, P. J. van der Spek, E. van Drunen-Schoenmaker, R. Kanaar, E. Lehmann, J. H. J. Hoeijmakers, and J. Kohli Rec8p, a Meiotic Recombination and Sister Chromatid Cohesion Phosphoprotein of the Rad21p Family Conserved from Fission Yeast to Humans Mol. Cell. Biol., May 1, 1999; 19(5): 3515 - 3528. [Abstract] [Full Text] [PDF]

				N. Kon, S. C. Schroeder, M. D. Krawchuk, and W. P. Wahls Regulation of the Mts1-Mts2-Dependent ade6-M26 Meiotic Recombination Hot Spot and Developmental Decisions by the Spc1 Mitogen-Activated Protein Kinase of Fission Yeast Mol. Cell. Biol., December 1, 1998; 18(12): 7575 - 7583. [Abstract] [Full Text]

				E. Hartsuiker, J. Bähler, and J. Kohli The Role of Topoisomerase II in Meiotic Chromosome Condensation and Segregation in Schizosaccharomyces pombe Mol. Biol. Cell, October 1, 1998; 9(10): 2739 - 2750. [Abstract] [Full Text]

				N. Kon, M. D. Krawchuk, B. G. Warren, G. R. Smith, and W. P. Wahls Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M26 meiotic recombination hotspot in pombe PNAS, December 9, 1997; 94(25): 13765 - 13770. [Abstract] [Full Text] [PDF]

				M. J. Moser, S. Y. Lee, R. E. Klevit, and T. N. Davis Ca[IMAGE] Binding to Calmodulin and Its Role in Schizosaccharomyces pombe as Revealed by Mutagenesis and NMR Spectroscopy J. Biol. Chem., September 1, 1995; 270(35): 20643 - 20652. [Abstract] [Full Text] [PDF]

				W P Wahls and G R Smith A heteromeric protein that binds to a meiotic homologous recombination hot spot: correlation of binding and hot spot activity. Genes & Dev., July 15, 1994; 8(14): 1693 - 1702. [Abstract] [PDF]

				L C DeVeaux and G R Smith Region-specific activators of meiotic recombination in Schizosaccharomyces pombe. Genes & Dev., January 15, 1994; 8(2): 203 - 210. [Abstract] [PDF]