Genetics, Vol 122, 823-835, Copyright © 1989

INVESTIGATIONS

Isolation and Characterization of Dominant Female Sterile Mutations of Drosophila melanogaster. II. Mutations on the Second Chromosome

J. Szabad, M. Erdelyi, G. Hoffmann, J. Szidonya and TRF. Wright
Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, H-6701, P.O. Box 521, Szeged, Hungary, Howard Hughes Medical Institute, Department of Biology, The University of Utah, Salt Lake City, Utah 84112

Twenty-four, second chromosome, dominant female sterile (Fs) mutations in Drosophila are described. Fs(2) were isolated at a frequency of approximately 1 per 1000 EMS-treated chromosomes screened. In comparison the isolation of frequency for second chromosome zygotic recessive lethal mutations was approximately 550 per 1000. Complementation analysis of the Fs(2) revertants showed that the 24 Fs(2) mutatations identify 13-15 loci, calculated to be about 65-75% of the second chromosome genes EMS mutable to dominant female sterility. Two of the Fs(2) mutations are useful tools for the dominant female sterile technique: Fs(2)1 for induction and detection of germ-line clones and Fs(2)Ugra for follicle cell clones. Several of the Fs(2) mutations bring about novel mutant phenotypes. Seven of them alter egg shape, whereas the others arrest development primarily at two stages: around fertilization by five Fs(2) and during cleavage divisions [by Fs(2) in three loci]. The remaining that allow development to the larval stage of differentiation include four new dorsal alleles and one dominant torso allele. Analysis of germ-line chimeras revealed that with two exceptions all the Fs(2) mutations are germ-line dependent. The Fs(2) mutations were mapped mainly on the basis of mitotic recombination induced in the female germ-line cells of adult females. That most of the Fs(2) may be gain-of-function mutations is indicated by the unusual behavior of the Fs(+) germ-line clones and also by the fact that 90% of the could be induced to revert.

This article has been cited by other articles:

				I. Gaspar and J. Szabad Glu415 in the {alpha}-tubulins plays a key role in stabilizing the microtubule-ADP-kinesin complexes J. Cell Sci., August 15, 2009; 122(16): 2857 - 2865. [Abstract] [Full Text] [PDF]

				T. Szalontai, I. Gaspar, I. Belecz, I. Kerekes, M. Erdelyi, I. Boros, and J. Szabad HorkaD, a Chromosome Instability-Causing Mutation in Drosophila, Is a Dominant-Negative Allele of lodestar Genetics, February 1, 2009; 181(2): 367 - 377. [Abstract] [Full Text] [PDF]

				Z. Venkei, I. Gaspar, G. Toth, and J. Szabad {alpha}4-Tubulin is involved in rapid formation of long microtubules to push apart the daughter centrosomes during earlyx Drosophila embryogenesis J. Cell Sci., August 1, 2006; 119(15): 3238 - 3248. [Abstract] [Full Text] [PDF]

				G. Timinszky, L. Tirian, F. T. Nagy, G. Toth, A. Perczel, Z. Kiss-Laszlo, I. Boros, P. R. Clarke, and J. Szabad The importin-{beta} P446L dominant-negative mutant protein loses RanGTP binding ability and blocks the formation of intact nuclear envelope J. Cell Sci., April 15, 2002; 115(8): 1675 - 1687. [Abstract] [Full Text] [PDF]

				S. Kuhfittig, J. Szabad, G. Schotta, J. Hoffmann, E. Mathe, and G. Reuter pitkinD, a Novel Gain-of-Function Enhancer of Position-Effect Variegation, Affects Chromatin Regulation During Oogenesis and Early Embryogenesis in Drosophila Genetics, March 1, 2001; 157(3): 1227 - 1244. [Abstract] [Full Text]

				M. Lippai, L. Tirian, I. Boros, J. Mihaly, M. Erdelyi, I. Belecz, E. Mathe, J. Posfai, A. Nagy, A. Udvardy, et al. The Ketel Gene Encodes a Drosophila Homologue of Importin-{beta} Genetics, December 1, 2000; 156(4): 1889 - 1900. [Abstract] [Full Text]

				L. Tirian, J. Puro, M. Erdelyi, I. Boros, B. Papp, M. Lippai, and J. Szabad The KetelD Dominant-Negative Mutations Identify Maternal Function of the Drosophila Importin-{beta} Gene Required for Cleavage Nuclei Formation Genetics, December 1, 2000; 156(4): 1901 - 1912. [Abstract] [Full Text]

				E Mathe, I Boros, K Josvay, K Li, J Puro, T. Kaufman, and J Szabad The Tomaj mutant alleles of alpha Tubulin67C reveal a requirement for the encoded maternal specific tubulin isoform in the sperm aster, the cleavage spindle apparatus and neurogenesis during embryonic development in Drosophila J. Cell Sci., January 4, 1998; 111(7): 887 - 896. [Abstract] [PDF]

				A Sapir, R Schweitzer, and B. Shilo Sequential activation of the EGF receptor pathway during Drosophila oogenesis establishes the dorsoventral axis Development, January 1, 1998; 125(2): 191 - 200. [Abstract] [PDF]

				M Reichman-Fried, B Dickson, E Hafen, and B Z Shilo Elucidation of the role of breathless, a Drosophila FGF receptor homolog, in tracheal cell migration. Genes & Dev., February 15, 1994; 8(4): 428 - 439. [Abstract] [PDF]

				T. B. Chou, E. Noll, and N. Perrimon Autosomal P[ovoD1] dominant female-sterile insertions in Drosophila and their use in generating germ-line chimeras Development, December 1, 1993; 119(4): 1359 - 1369. [Abstract] [PDF]

				W Sullivan, P Fogarty, and W Theurkauf Mutations affecting the cytoskeletal organization of syncytial Drosophila embryos Development, January 8, 1993; 118(4): 1245 - 1254. [Abstract] [PDF]

				B Dickson, F Sprenger, and E Hafen Prepattern in the developing Drosophila eye revealed by an activated torso--sevenless chimeric receptor. Genes & Dev., December 1, 1992; 6(12a): 2327 - 2339. [Abstract] [PDF]