- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Weber, D.
- Articles by Helentjaris, T.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Weber, D.
- Articles by Helentjaris, T.
Genetics, Vol 121, 583-590, Copyright © 1989
INVESTIGATIONS |
Mapping RFLP Loci in Maize Using B-A Translocations
D. Weber and T. Helentjaris
Genetics Group, Department of Biological Sciences, Illinois State University, Normal, Illinois 61761
Plants hypoploid for specific segments of each of the maize (Zea mays L.) chromosomes were generated using 24 different B-A translocations. Plants carrying each of the B-A translocations were crossed as male parents to inbreds, and sibling progeny hypoploid or not hypoploid for specific chromosomal segments were recovered. Genomic DNAs from the parents, hypoploid progeny, and nonhypoploid (euploid or hyperploid) progeny for each of these B-A translocations were digested with restriction enzymes, electrophoresed in agarose gels, blotted onto reusable nylon membranes, and probed with nick-translated, cloned DNA fragments which had been mapped previously by restriction fragment length polymorphism (RFLP) analysis to the chromosome involved in the B-A translocation. The chromosomal segment on our RFLP map which was uncovered by each of the - B-A translocations was determined. This work unequivocally identified the short and long arms of each chromosome on this map, and it also identified the region on each chromosome which contains the centromere. Because the breakpoints of the B-A translocations were previously known on the cytological and the conventional genetic maps, this study also allowed this RFLP map to be more highly correlated with these maps.
This article has been cited by other articles:
 |
|
 |
|
  R. J. Okagaki, M. S. Jacobs, A. O. Stec, R. G. Kynast, E. Buescher, H. W. Rines, M. I. Vales, O. Riera-Lizarazu, M. Schneerman, G. Doyle, et al. Maize Centromere Mapping: A Comparison of Physical and Genetic Strategies J. Hered., March 1, 2008; 99(2): 85 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Luce, A. Sharma, O. S. B. Mollere, T. K. Wolfgruber, K. Nagaki, J. Jiang, G. G. Presting, and R. K. Dawe Precise Centromere Mapping Using a Combination of Repeat Junction Markers and Chromatin Immunoprecipitation-Polymerase Chain Reaction Genetics, October 1, 2006; 174(2): 1057 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-J. R. Wang, L. Harper, and W. Z. Cande High-Resolution Single-Copy Gene Fluorescence in Situ Hybridization and Its Use in the Construction of a Cytogenetic Map of Maize Chromosome 9 PLANT CELL, March 1, 2006; 18(3): 529 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Anderson, N. Salameh, H. W. Bass, L. C. Harper, W. Z. Cande, G. Weber, and S. M. Stack Integrating Genetic Linkage Maps With Pachytene Chromosome Structure in Maize Genetics, April 1, 2004; 166(4): 1923 - 1933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Cheng, G. G. Presting, C. R. Buell, R. A. Wing, and J. Jiang High-Resolution Pachytene Chromosome Mapping of Bacterial Artificial Chromosomes Anchored by Genetic Markers Reveals the Centromere Location and the Distribution of Genetic Recombination Along Chromosome 10 of Rice Genetics, April 1, 2001; 157(4): 1749 - 1757. [Abstract] [Full Text]
|
|
|
|
|
|
G. L. Davis, M. D. McMullen, C. Baysdorfer, T. Musket, D. Grant, M. Staebell, G. Xu, M. Polacco, L. Koster, S. Melia-Hancock, et al. A Maize Map Standard With Sequenced Core Markers, Grass Genome Reference Points and 932 Expressed Sequence Tagged Sites (ESTs) in a 1736-Locus Map Genetics, July 1, 1999; 152(3): 1137 - 1172. [Abstract] [Full Text]
|
|