- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Flessel, M. C.
- Articles by Thorner, J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Flessel, M. C.
- Articles by Thorner, J.
Genetics, Vol 121, 223-236, Copyright © 1989
INVESTIGATIONS |
The MF{alpha}1 Gene of Saccharomyces cerevisiae: Genetic Mapping and Mutational Analysis of Promoter Elements
M. C. Flessel, A. J. Brake and J. Thorner
Graduate Group in Microbiology, University of California, Berkeley, California 94720 Current address: Department of Molecular Biology, University of California, Berkeley, California 94720.
The activity and cell-type specificity of the promoter of the MF{alpha}1 gene of Saccharomyces cerevisiae were examined by measuring expression of an MF{alpha}1-SUC2 gene fusion in MATa, MAT{alpha}, and MATa/MAT{alpha} cells. A high level of invertase activity was observed only in MAT{alpha} cells. Weak expression occurred in MATa cells when the hybrid gene was carried on a multicopy plasmid or on a centromere-containing plasmid, but not when the hybrid gene was integrated at the normal MF{alpha}1 locus. Analysis of a set of 5'-deletions of the promoter region of the MF{alpha}1-SUC2 gene on the multicopy plasmid indicated that sequences from -354 to -274 upstream of the translational start site were required for high level expression in MAT{alpha} cells. Smaller internal deletions and insertions within the promoter region of the MF{alpha}1-SUC2 gene were inserted into the genome at the normal MF{alpha}1 locus. These mutations further delineated four promoter domains important for expression: (1) two 26 bp elements (-365 to -340 and -312 to -287) with imperfect dyad symmetry; (2) a 40 bp segment (-264 to -226) that lies about 120 bp upstream of the TATA box; and (3) the TATA box itself (-128 to -122). The transcriptional start sites of the normal MF{alpha}1 promoter and of a mutant lacking the TATA box were determined. The MF{alpha}1 locus was mapped to the left arm of chromosome XVI, about 22 cM centromere-proximal to the PEP4 gene.
This article has been cited by other articles:
 |
|
 |
|
  O. A. Zill and J. Rine Interspecies variation reveals a conserved repressor of {alpha}-specific genes in Saccharomyces yeasts Genes & Dev., June 15, 2008; 22(12): 1704 - 1716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Panwar, M. Legrand, D. Dignard, M. Whiteway, and Paul. T. Magee MF{alpha}1, the Gene Encoding the {alpha} Mating Pheromone of Candida albicans Eukaryot. Cell, December 1, 2003; 2(6): 1350 - 1360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y O Yuan, I L Stroke, and S Fields Coupling of cell identity to signal response in yeast: interaction between the alpha 1 and STE12 proteins. Genes & Dev., August 1, 1993; 7(8): 1584 - 1597. [Abstract] [PDF]
|
|
|
|
|
|
G Ammerer Identification, purification, and cloning of a polypeptide (PRTF/GRM) that binds to mating-specific promoter elements in yeast. Genes & Dev., February 1, 1990; 4(2): 299 - 312. [Abstract] [PDF]
|
|
|
|
|
|
E E Jarvis, K L Clark, and G F Sprague The yeast transcription activator PRTF, a homolog of the mammalian serum response factor, is encoded by the MCM1 gene. Genes & Dev., July 1, 1989; 3(7): 936 - 945. [Abstract] [PDF]
|
|