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Genetics, Vol 121, 5-12, Copyright © 1989
INVESTIGATIONS |
Phage {lambda} Cro Protein and cI Repressor Use Two Different Patterns of Specific Protein-DNA Interactions to Achieve Sequence Specificity in Vivo
N. Benson and P. Youderian
Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-1481
By assaying the binding of wild-type Cro to a set of 40 mutant {lambda} operators in vivo, we have determined that the 14 outermost base pairs of the 17 base pair, consensus {lambda} operator are critical for Cro binding. Cro protein recognizes 4 base pairs in a {lambda} operator half-site in different ways than cI repressor. The sequence determinants of Cro binding at these critical positions in vivo are nearly perfectly consistent with the model proposed by W. F. ANDERSON, D. H. OHLENDORF, Y. TAKEDA and B. W. MATTHEWS and modified by Y. TAKEDA, A. SARAI and V. M. RIVERA for the specific interactions between Cro and its operator, and explain the relative order of affinities of the six natural {lambda} operators for Cro. Our data call into question the idea that {lambda} repressor and Cro protein recognize the consensus {lambda} operator by nearly identical patterns of specific interactions.