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Genetics, Vol 120, 1053-1059, Copyright © 1988
INVESTIGATIONS |
Rex-Induced Recombination Implies Bipolar Organization of the Ribosomal RNA Genes of Drosophila melanogaster
L. G. Robbins and E. E. Swanson
Department of Zoology and Genetics Program, Michigan State University, East Lansing, Michigan 48824
Rex-induced mitotic recombination was used to determine whether nucleolus organizers can pair in both inverted and noninverted orientations. Two target chromosomes, each duplicated for the rDNA region, were exposed to maternal Rex activity. Recombination in one orientation should yield deletion of the material between the two nucleolus organizers, recombination in the other orientation should yield inversion of the same material. Both products were recovered from both target chromosomes. The generality of using Rex-mediated recombination for analysis of the rDNA is considered.
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