Genetics, Vol 120, 621-623, Copyright © 1988

INVESTIGATIONS

Genetic Applications of an Inverse Polymerase Chain Reaction

H. Ochman, A. S. Gerber and D. L. Hartl
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110

A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.


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 Nucleic Acids ResHome page
G. Tan, Y. Gao, M. Shi, X. Zhang, S. He, Z. Chen, and C. An
SiteFinding-PCR: a simple and efficient PCR method for chromosome walking
Nucleic Acids Res., August 2, 2005; 33(13): e122 - e122.
[Abstract] [Full Text] [PDF]

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 GeneticsHome page
L. M. Matzkin, T. J. S. Merritt, C.-T. Zhu, and W. F. Eanes
The Structure and Population Genetics of the Breakpoints Associated With the Cosmopolitan Chromosomal Inversion In(3R)Payne in Drosophila melanogaster
Genetics, July 1, 2005; 170(3): 1143 - 1152.
[Abstract] [Full Text] [PDF]

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 J. Bacteriol.Home page
K. Bailey-Smith, S. J. Todd, T. W. Southworth, J. Proctor, and A. Moir
The ExsA Protein of Bacillus cereus Is Required for Assembly of Coat and Exosporium onto the Spore Surface
J. Bacteriol., June 1, 2005; 187(11): 3800 - 3806.
[Abstract] [Full Text] [PDF]

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 Proc. Natl. Acad. Sci. USAHome page
E. S. Kandel, T. Lu, Y. Wan, M. K. Agarwal, M. W. Jackson, and G. R. Stark
Mutagenesis by reversible promoter insertion to study the activation of NF-{kappa}B
PNAS, May 3, 2005; 102(18): 6425 - 6430.
[Abstract] [Full Text] [PDF]

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 Proc. Natl. Acad. Sci. USAHome page
S. B. Corley, R. Carpenter, L. Copsey, and E. Coen
Floral asymmetry involves an interplay between TCP and MYB transcription factors in Antirrhinum
PNAS, April 5, 2005; 102(14): 5068 - 5073.
[Abstract] [Full Text] [PDF]

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 MicrobiologyHome page
W. Klomklang, A. Tani, K. Kimbara, R. Mamoto, T. Ueda, M. Shimao, and F. Kawai
Biochemical and molecular characterization of a periplasmic hydrolase for oxidized polyvinyl alcohol from Sphingomonas sp. strain 113P3
Microbiology, April 1, 2005; 151(4): 1255 - 1262.
[Abstract] [Full Text] [PDF]

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 J. Virol.Home page
K. Fujii, Y. Fujii, T. Noda, Y. Muramoto, T. Watanabe, A. Takada, H. Goto, T. Horimoto, and Y. Kawaoka
Importance of both the Coding and the Segment-Specific Noncoding Regions of the Influenza A Virus NS Segment for Its Efficient Incorporation into Virions
J. Virol., March 15, 2005; 79(6): 3766 - 3774.
[Abstract] [Full Text] [PDF]

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 J. Bacteriol.Home page
Y. Nakaguchi and M. Nishibuchi
The Promoter Region Rather than Its Downstream Inverted Repeat Sequence Is Responsible for Low-Level Transcription of the Thermostable Direct Hemolysin-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus
J. Bacteriol., March 1, 2005; 187(5): 1849 - 1855.
[Abstract] [Full Text] [PDF]

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 J. Bacteriol.Home page
G. Huang, Q. Fan, S. Lechno-Yossef, E. Wojciuch, C. P. Wolk, T. Kaneko, and S. Tabata
Clustered Genes Required for the Synthesis of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120
J. Bacteriol., February 1, 2005; 187(3): 1114 - 1123.
[Abstract] [Full Text] [PDF]

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 GeneticsHome page
K. Naito, M. Kusaba, N. Shikazono, T. Takano, A. Tanaka, T. Tanisaka, and M. Nishimura
Transmissible and Nontransmissible Mutations Induced by Irradiating Arabidopsis thaliana Pollen With {gamma}-Rays and Carbon Ions
Genetics, February 1, 2005; 169(2): 881 - 889.
[Abstract] [Full Text] [PDF]

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 GeneticsHome page
J. M. Kolkman, L. J. Conrad, P. R. Farmer, K. Hardeman, K. R. Ahern, P. E. Lewis, R. J. H. Sawers, S. Lebejko, P. Chomet, and T. P. Brutnell
Distribution of Activator (Ac) Throughout the Maize Genome for Use in Regional Mutagenesis
Genetics, February 1, 2005; 169(2): 981 - 995.
[Abstract] [Full Text] [PDF]

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 Plant Cell PhysiolHome page
G. An, S. Lee, S.-H. Kim, and S.-R. Kim
Molecular Genetics Using T-DNA in Rice
Plant Cell Physiol., January 15, 2005; 46(1): 14 - 22.
[Abstract] [Full Text] [PDF]

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 Proc. Natl. Acad. Sci. USAHome page
C. Meyer, B. Schneider, M. Reichel, S. Angermueller, S. Strehl, S. Schnittger, C. Schoch, M. W. J. C. Jansen, J. J. van Dongen, R. Pieters, et al.
Diagnostic tool for the identification of MLL rearrangements including unknown partner genes
PNAS, January 11, 2005; 102(2): 449 - 454.
[Abstract] [Full Text] [PDF]

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 Appl. Environ. Microbiol.Home page
P. J. Johnsen, J. I. Osterhus, H. Sletvold, M. Sorum, H. Kruse, K. Nielsen, G. S. Simonsen, and A. Sundsfjord
Persistence of Animal and Human Glycopeptide-Resistant Enterococci on Two Norwegian Poultry Farms Formerly Exposed to Avoparcin Is Associated with a Widespread Plasmid-Mediated vanA Element within a Polyclonal Enterococcus faecium Population
Appl. Envir. Microbiol., January 1, 2005; 71(1): 159 - 168.
[Abstract] [Full Text] [PDF]

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 Plant Cell PhysiolHome page
H. Pyo, T. Demura, and H. Fukuda
Spatial and Temporal Tracing of Vessel Differentiation in Young Arabidopsis Seedlings by the Expression of an Immature Tracheary Element-specific Promoter
Plant Cell Physiol., October 15, 2004; 45(10): 1529 - 1536.
[Abstract] [Full Text] [PDF]

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 Genes Dev.Home page
T. Yang, D. Liang, P. J. Koch, D. Hohl, F. Kheradmand, and P. A. Overbeek
Epidermal detachment, desmosomal dissociation, and destabilization of corneodesmosin in Spink5-/- mice
Genes & Dev., October 1, 2004; 18(19): 2354 - 2358.
[Abstract] [Full Text] [PDF]

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 J. Virol.Home page
K. Iwatsuki-Horimoto, T. Horimoto, Y. Fujii, and Y. Kawaoka
Generation of Influenza A Virus NS2 (NEP) Mutants with an Altered Nuclear Export Signal Sequence
J. Virol., September 15, 2004; 78(18): 10149 - 10155.
[Abstract] [Full Text] [PDF]

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 Appl. Environ. Microbiol.Home page
N. Nakashima and T. Tamura
Isolation and Characterization of a Rolling-Circle-Type Plasmid from Rhodococcus erythropolis and Application of the Plasmid to Multiple-Recombinant-Protein Expression
Appl. Envir. Microbiol., September 1, 2004; 70(9): 5557 - 5568.
[Abstract] [Full Text] [PDF]

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 Mol. Cell. Biol.Home page
E. D. Ross, U. Baxa, and R. B. Wickner
Scrambled Prion Domains Form Prions and Amyloid
Mol. Cell. Biol., August 15, 2004; 24(16): 7206 - 7213.
[Abstract] [Full Text] [PDF]

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 J. Bacteriol.Home page
A. O. Kilic, L. Tao, Y. Zhang, Y. Lei, A. Khammanivong, and M. C. Herzberg
Involvement of Streptococcus gordonii Beta-Glucoside Metabolism Systems in Adhesion, Biofilm Formation, and In Vivo Gene Expression
J. Bacteriol., July 1, 2004; 186(13): 4246 - 4253.
[Abstract] [Full Text] [PDF]

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 Mol. Endocrinol.Home page
S. Frankton, C. B. Harvey, L. M. Gleason, A. Fadel, and G. R. Williams
Multiple Messenger Ribonucleic Acid Variants Regulate Cell-Specific Expression of Human Thyroid Hormone Receptor {beta}1
Mol. Endocrinol., July 1, 2004; 18(7): 1631 - 1642.
[Abstract] [Full Text] [PDF]

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 J. Bacteriol.Home page
V. David, B. Bozdogan, J.-L. Mainardi, R. Legrand, L. Gutmann, and R. Leclercq
Mechanism of Intrinsic Resistance to Vancomycin in Clostridium innocuum NCIB 10674
J. Bacteriol., June 1, 2004; 186(11): 3415 - 3422.
[Abstract] [Full Text] [PDF]

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 J Med MicrobiolHome page
T. Song, C. Toma, N. Nakasone, and M. Iwanaga
Aerolysin is activated by metalloprotease in Aeromonas veronii biovar sobria
J. Med. Microbiol., June 1, 2004; 53(6): 477 - 482.
[Abstract] [Full Text] [PDF]

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 J. Bacteriol.Home page
M. Blokesch, M. Rohrmoser, S. Rode, and A. Bock
HybF, a Zinc-Containing Protein Involved in NiFe Hydrogenase Maturation
J. Bacteriol., May 1, 2004; 186(9): 2603 - 2611.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
M.-M. Sampaio, F. Chevance, R. Dippel, T. Eppler, A. Schlegel, W. Boos, Y.-J. Lu, and C. O. Rock
Phosphotransferase-mediated Transport of the Osmolyte 2-O-{alpha}-Mannosyl-D-glycerate in Escherichia coli Occurs by the Product of the mngA (hrsA) Gene and Is Regulated by the mngR (farR) Gene Product Acting as Repressor
J. Biol. Chem., February 13, 2004; 279(7): 5537 - 5548.
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 Eukaryot CellHome page
J. S. Fillingham, T. A. Thing, N. Vythilingum, A. Keuroghlian, D. Bruno, G. B. Golding, and R. E. Pearlman
A Non-Long Terminal Repeat Retrotransposon Family Is Restricted to the Germ Line Micronucleus of the Ciliated Protozoan Tetrahymena thermophila
Eukaryot. Cell, February 1, 2004; 3(1): 157 - 169.
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 GeneticsHome page
X. Yu and A. Gabriel
Reciprocal Translocations in Saccharomyces cerevisiae Formed by Nonhomologous End Joining
Genetics, February 1, 2004; 166(2): 741 - 751.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
G. Cecchetto, S. Amillis, G. Diallinas, C. Scazzocchio, and C. Drevet
The AzgA Purine Transporter of Aspergillus nidulans: CHARACTERIZATION OF A PROTEIN BELONGING TO A NEW PHYLOGENETIC CLUSTER
J. Biol. Chem., January 30, 2004; 279(5): 3132 - 3141.
[Abstract] [Full Text] [PDF]