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Genetics, Vol 120, 621-623, Copyright © 1988
INVESTIGATIONS |
Genetic Applications of an Inverse Polymerase Chain Reaction
H. Ochman, A. S. Gerber and D. L. Hartl
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110
A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.
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Y. Nakaguchi and M. Nishibuchi The Promoter Region Rather than Its Downstream Inverted Repeat Sequence Is Responsible for Low-Level Transcription of the Thermostable Direct Hemolysin-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus J. Bacteriol., March 1, 2005; 187(5): 1849 - 1855. [Abstract] [Full Text] [PDF] |
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G. Huang, Q. Fan, S. Lechno-Yossef, E. Wojciuch, C. P. Wolk, T. Kaneko, and S. Tabata Clustered Genes Required for the Synthesis of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120 J. Bacteriol., February 1, 2005; 187(3): 1114 - 1123. [Abstract] [Full Text] [PDF] |
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K. Naito, M. Kusaba, N. Shikazono, T. Takano, A. Tanaka, T. Tanisaka, and M. Nishimura Transmissible and Nontransmissible Mutations Induced by Irradiating Arabidopsis thaliana Pollen With {gamma}-Rays and Carbon Ions Genetics, February 1, 2005; 169(2): 881 - 889. [Abstract] [Full Text] [PDF] |
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J. M. Kolkman, L. J. Conrad, P. R. Farmer, K. Hardeman, K. R. Ahern, P. E. Lewis, R. J. H. Sawers, S. Lebejko, P. Chomet, and T. P. Brutnell Distribution of Activator (Ac) Throughout the Maize Genome for Use in Regional Mutagenesis Genetics, February 1, 2005; 169(2): 981 - 995. [Abstract] [Full Text] [PDF] |
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G. An, S. Lee, S.-H. Kim, and S.-R. Kim Molecular Genetics Using T-DNA in Rice Plant Cell Physiol., January 15, 2005; 46(1): 14 - 22. [Abstract] [Full Text] [PDF] |
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C. Meyer, B. Schneider, M. Reichel, S. Angermueller, S. Strehl, S. Schnittger, C. Schoch, M. W. J. C. Jansen, J. J. van Dongen, R. Pieters, et al. Diagnostic tool for the identification of MLL rearrangements including unknown partner genes PNAS, January 11, 2005; 102(2): 449 - 454. [Abstract] [Full Text] [PDF] |
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P. J. Johnsen, J. I. Osterhus, H. Sletvold, M. Sorum, H. Kruse, K. Nielsen, G. S. Simonsen, and A. Sundsfjord Persistence of Animal and Human Glycopeptide-Resistant Enterococci on Two Norwegian Poultry Farms Formerly Exposed to Avoparcin Is Associated with a Widespread Plasmid-Mediated vanA Element within a Polyclonal Enterococcus faecium Population Appl. Envir. Microbiol., January 1, 2005; 71(1): 159 - 168. [Abstract] [Full Text] [PDF] |
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H. Pyo, T. Demura, and H. Fukuda Spatial and Temporal Tracing of Vessel Differentiation in Young Arabidopsis Seedlings by the Expression of an Immature Tracheary Element-specific Promoter Plant Cell Physiol., October 15, 2004; 45(10): 1529 - 1536. [Abstract] [Full Text] [PDF] |
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T. Yang, D. Liang, P. J. Koch, D. Hohl, F. Kheradmand, and P. A. Overbeek Epidermal detachment, desmosomal dissociation, and destabilization of corneodesmosin in Spink5-/- mice Genes & Dev., October 1, 2004; 18(19): 2354 - 2358. [Abstract] [Full Text] [PDF] |
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K. Iwatsuki-Horimoto, T. Horimoto, Y. Fujii, and Y. Kawaoka Generation of Influenza A Virus NS2 (NEP) Mutants with an Altered Nuclear Export Signal Sequence J. Virol., September 15, 2004; 78(18): 10149 - 10155. [Abstract] [Full Text] [PDF] |
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N. Nakashima and T. Tamura Isolation and Characterization of a Rolling-Circle-Type Plasmid from Rhodococcus erythropolis and Application of the Plasmid to Multiple-Recombinant-Protein Expression Appl. Envir. Microbiol., September 1, 2004; 70(9): 5557 - 5568. [Abstract] [Full Text] [PDF] |
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E. D. Ross, U. Baxa, and R. B. Wickner Scrambled Prion Domains Form Prions and Amyloid Mol. Cell. Biol., August 15, 2004; 24(16): 7206 - 7213. [Abstract] [Full Text] [PDF] |
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A. O. Kilic, L. Tao, Y. Zhang, Y. Lei, A. Khammanivong, and M. C. Herzberg Involvement of Streptococcus gordonii Beta-Glucoside Metabolism Systems in Adhesion, Biofilm Formation, and In Vivo Gene Expression J. Bacteriol., July 1, 2004; 186(13): 4246 - 4253. [Abstract] [Full Text] [PDF] |
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S. Frankton, C. B. Harvey, L. M. Gleason, A. Fadel, and G. R. Williams Multiple Messenger Ribonucleic Acid Variants Regulate Cell-Specific Expression of Human Thyroid Hormone Receptor {beta}1 Mol. Endocrinol., July 1, 2004; 18(7): 1631 - 1642. [Abstract] [Full Text] [PDF] |
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V. David, B. Bozdogan, J.-L. Mainardi, R. Legrand, L. Gutmann, and R. Leclercq Mechanism of Intrinsic Resistance to Vancomycin in Clostridium innocuum NCIB 10674 J. Bacteriol., June 1, 2004; 186(11): 3415 - 3422. [Abstract] [Full Text] [PDF] |
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T. Song, C. Toma, N. Nakasone, and M. Iwanaga Aerolysin is activated by metalloprotease in Aeromonas veronii biovar sobria J. Med. Microbiol., June 1, 2004; 53(6): 477 - 482. [Abstract] [Full Text] [PDF] |
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M. Blokesch, M. Rohrmoser, S. Rode, and A. Bock HybF, a Zinc-Containing Protein Involved in NiFe Hydrogenase Maturation J. Bacteriol., May 1, 2004; 186(9): 2603 - 2611. [Abstract] [Full Text] [PDF] |
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M.-M. Sampaio, F. Chevance, R. Dippel, T. Eppler, A. Schlegel, W. Boos, Y.-J. Lu, and C. O. Rock Phosphotransferase-mediated Transport of the Osmolyte 2-O-{alpha}-Mannosyl-D-glycerate in Escherichia coli Occurs by the Product of the mngA (hrsA) Gene and Is Regulated by the mngR (farR) Gene Product Acting as Repressor J. Biol. Chem., February 13, 2004; 279(7): 5537 - 5548. [Abstract] [Full Text] [PDF] |
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J. S. Fillingham, T. A. Thing, N. Vythilingum, A. Keuroghlian, D. Bruno, G. B. Golding, and R. E. Pearlman A Non-Long Terminal Repeat Retrotransposon Family Is Restricted to the Germ Line Micronucleus of the Ciliated Protozoan Tetrahymena thermophila Eukaryot. Cell, February 1, 2004; 3(1): 157 - 169. [Abstract] [Full Text] [PDF] |
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X. Yu and A. Gabriel Reciprocal Translocations in Saccharomyces cerevisiae Formed by Nonhomologous End Joining Genetics, February 1, 2004; 166(2): 741 - 751. [Abstract] [Full Text] [PDF] |
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G. Cecchetto, S. Amillis, G. Diallinas, C. Scazzocchio, and C. Drevet The AzgA Purine Transporter of Aspergillus nidulans: CHARACTERIZATION OF A PROTEIN BELONGING TO A NEW PHYLOGENETIC CLUSTER J. Biol. Chem., January 30, 2004; 279(5): 3132 - 3141. [Abstract] [Full Text] [PDF] |
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