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Genetics, Vol 120, 533-543, Copyright © 1988
INVESTIGATIONS |
Genetic Analysis of Mouse t Haplotypes Using Mutations Induced by Ethylnitrosourea Mutagenesis: The Order of T and qk Is Inverted in t Mutants
M. J. Justice and V. C. Bode
Division of Biology, Kansas State University, Manhattan, Kansas 66506
The t region of mouse chromosome 17 exhibits recombination suppression with wild-type chromatin. However, the region has resisted classical genetic dissection because of a lack of defined variants. Mutations induced by N-ethyl-N-nitrosourea (ENU) at the Brachyury (T), quaking (qk), and tufted (tf) loci of the mouse t(w5) haplotype have now allowed the analysis of crossovers between two complete t haplotypes. A classical breeding analysis of the complete t haplotypes, t(w5) and t(12), utilizing the newly induced markers, reveals two inversions in t chromatin: one involving T and qk, and one involving tf and the H-2 complex. Moreover, the recombination frequency between the loci of T and qk is reduced compared to the frequency reported in normal chromatin. These two inversions are a sufficient explanation for the recombination inhibition with normal chromatin exhibited by t haplotypes isolated from the wild. Furthermore, the reduced recombination frequency between T and qk may indicate that the proximal gene rearrangement is not a simple inversion.
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