- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Zolan, M. E.
- Articles by Pukkila, P. J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Zolan, M. E.
- Articles by Pukkila, P. J.
Genetics, Vol 120, 379-387, Copyright © 1988
INVESTIGATIONS |
Production and Characterization of Radiation-Sensitive Meiotic Mutants of Coprinus cinereus
M. E. Zolan, C. J. Tremel and P. J. Pukkila
Department of Natural Sciences, University of Michigan-Dearborn, Dearborn, Michigan 48128 To whom communications should be sent.
We have isolated four {gamma}-ray-sensitive mutants of the basidiomycete Coprinus cinereus. When homozygous, two of these (rad 3-1 and rad 9-1) produce fruiting bodies with very few viable basidiospores, the products of meiosis in this organism. A less radiation-sensitive allele of RAD 3, rad 3-2, causes no apparent meiotic defect in homozygous strains. Quantitative measurements of oidial survival of rad 3-1;rad 9-1 double mutants compared to the single mutants indicated that rad 3-1 and rad 9-1 mutants are defective in the same DNA repair pathway. In the few viable basidiospores that are produced by these two strains, essentially normal levels of meiotic recombination can be detected. None of the mutants exhibits increased sensitivity to UV radiation. Cytological examination of meiotic chromosomes from mutant and wild-type fruiting bodies showed that rad 3-1 homozygous strains fail to condense and pair homologous chromosomes during prophase I. Although rad 9-1 strains are successful at chromosome pairing, meiosis is usually not completed in these mutants.
This article has been cited by other articles:
 |
|
 |
|
  W. J. Cummings, S. T. Merino, K. G. Young, L. Li, C. W. Johnson, E. A. Sierra, and M. E. Zolan The Coprinus cinereus adherin Rad9 functions in Mre11-dependent DNA repair, meiotic sister-chromatid cohesion, and meiotic homolog pairing PNAS, November 12, 2002; 99(23): 14958 - 14963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Merino, W. J. Cummings, S. N. Acharya, and M. E. Zolan Replication-dependent early meiotic requirement for Spo11 and Rad50 PNAS, September 5, 2000; (2000) 190346097. [Abstract] [Full Text]
|
|
|
|
 |
|
 U. Kues Life History and Developmental Processes in the Basidiomycete Coprinus cinereus Microbiol. Mol. Biol. Rev., June 1, 2000; 64(2): 316 - 353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. Gerecke and M. E. Zolan An mre11 Mutant of Coprinus cinereus Has Defects in Meiotic Chromosome Pairing, Condensation and Synapsis Genetics, March 1, 2000; 154(3): 1125 - 1139. [Abstract] [Full Text]
|
|
|
|
|
|
S. F. O'Shea, P. T. Chaure, J. R. Halsall, N. S. Olesnicky, A. Leibbrandt, I. F. Connerton, and L. A. Casselton A Large Pheromone and Receptor Gene Complex Determines Multiple B Mating Type Specificities in Coprinus cinereus Genetics, March 1, 1998; 148(3): 1081 - 1090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Merino, W. J. Cummings, S. N. Acharya, and M. E. Zolan Replication-dependent early meiotic requirement for Spo11 and Rad50 PNAS, September 12, 2000; 97(19): 10477 - 10482. [Abstract] [Full Text] [PDF]
|
|