Different Types of Recombination Events Are Controlled by the RAD1 and RAD52 Genes of Saccharomyces cerevisiae

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Different Types of Recombination Events Are Controlled by the RAD1 and RAD52 Genes of Saccharomyces cerevisiae

H. L. Klein
Department of Biochemistry, New York University, New York, New York 10016

Intrachromosomal recombination within heteroallelic duplications located on chromosomes III and XV of Saccharomyces cerevisae has been examined. Both possible orientations of alleles have been used in each duplication. Three recombinant classes, gene conversions, pop-outs and triplications, were recovered. Some of the recombinant classes were not anticipated from the particular allele orientation of the duplication. Recovery of these unexpected recombinants requires the RAD1 gene. These studies show that RAD1 has a role in recombination between repeated sequences, and that the recombination event is a gene conversion associated with a crossover. These events appear to involve very localized conversion of a heteroduplex region and are distinct from RAD52 mediated gene conversion events. Evidence is also presented to suggest that most recombination events between direct repeats are intrachromatid, not between sister chromatids.

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				A. J. Rattray, B. K. Shafer, and D. J. Garfinkel The Saccharomyces cerevisiae DNA Recombination and Repair Functions of the RAD52 Epistasis Group Inhibit Ty1 Transposition Genetics, February 1, 2000; 154(2): 543 - 556. [Abstract] [Full Text]

				N. Erdeniz and R. Rothstein Rsp5, a Ubiquitin-Protein Ligase, Is Involved in Degradation of the Single-Stranded-DNA Binding Protein Rfa1 in Saccharomyces cerevisiae Mol. Cell. Biol., January 1, 2000; 20(1): 224 - 232. [Abstract] [Full Text]

				F. Paques and J. E. Haber Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 349 - 404. [Abstract] [Full Text] [PDF]

				J. Smith and R. Rothstein An Allele of RFA1 Suppresses RAD52-Dependent Double-Strand Break Repair in Saccharomyces cerevisiae Genetics, February 1, 1999; 151(2): 447 - 458. [Abstract] [Full Text]

				T. Sugiyama, J. H. New, and S. C. Kowalczykowski DNA annealing by Rad52 Protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA PNAS, May 26, 1998; 95(11): 6049 - 6054. [Abstract] [Full Text] [PDF]

				R. G. Sargent, R. L. Rolig, A. E. Kilburn, G. M. Adair, J. H. Wilson, and R. S. Nairn Recombination-dependent deletion formation in mammalian cells deficient in the nucleotide excision repair gene ERCC1 PNAS, November 25, 1997; 94(24): 13122 - 13127. [Abstract] [Full Text] [PDF]

				J. E. Haber and W.-Y. Leung Lack of chromosome territoriality in yeast: Promiscuous rejoining of broken chromosome ends PNAS, November 26, 1996; 93(24): 13949 - 13954. [Abstract] [Full Text] [PDF]

				A. A. Davies, E. C. Friedberg, A. E. Tomkinson, R. D. Wood, and S. C. West Role of the Rad1 and Rad10 Proteins in Nucleotide Excision Repair and Recombination J. Biol. Chem., October 20, 1995; 270(42): 24638 - 24641. [Abstract] [Full Text] [PDF]

				A. Bardwell, L Bardwell, A. Tomkinson, and E. Friedberg Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease Science, September 30, 1994; 265(5181): 2082 - 2085. [Abstract] [PDF]

				J Fishman-Lobell and J. Haber Removal of nonhomologous DNA ends in double-strand break recombination: the role of the yeast ultraviolet repair gene RAD1 Science, October 16, 1992; 258(5081): 480 - 484. [Abstract] [PDF]