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Genetics, Vol 120, 7-21, Copyright © 1988
INVESTIGATIONS |
Chain-Bias of Escherichia coli Rec-Mediated {lambda} Patch Recombinants Is Independent of the Orientation of {lambda} cos
S. M. Rosenberg
Present address: Department of Biochemistry, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, Utah 84132.
Chi is a hotspot for homologous recombination mediated by the RecBCD (Rec) pathway of Escherichia coli. For Rec-mediated recombination of phage {lambda}, the orientation of {lambda} cos in the {lambda} chromosome dictates the direction of travel of RecBCD enzyme through DNA and dictates which orientation of Chi or Chi-like sequences will be active in stimulating recombination. I previously found that Rec-mediated {lambda} patch heteroduplexes, stimulated by Chi or not, are chain-biased; at the {lambda} P locus, recombinant information resides on the {lambda} r chain. This bias exists in the presence or absence of Chi sites. Reported herein is the finding that r-chain-bias at the P locus is independent of the orientation of {lambda} cos and thus also independent of the orientation of active Chi's or Chi-like sequences and of the direction of travel of RecBCD enzyme. These results disprove previously elaborated models in which a chain-specific nick at Chi initiates recombination, and imply that some other chain-distinguishing process is involved with recombination. Replication and transcription are candidates for such a process.
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