Genetics, Vol 119, 579-593, Copyright © 1988

INVESTIGATIONS

Genetic Analysis of Chromosomal Region 67A-D of Drosophila melanogaster

B. G. Leicht and J. J. Bonner
Present address: Department of Molecular Genetics, 484 W. 12th Avenue, The Ohio State University, Columbus, Ohio 43210.

In an effort to (1) characterize the 67 interval of chromosome 3 of Drosophila melanogaster genetically and (2) isolate mutations of the 67B1 small heat shock protein (hsp) gene cluster specifically, we undertook a mutational analysis of the 67A-D subinterval. Using a deficiency of the 67A2 to 67D11-13 region, Df(3L)AC1, we screened 8700 diepoxybutane-treated chromosomes and 7800 ethyl methanesulfonate-treated chromosomes for visible and lethal mutations throughout this interval and recovered 74 independent recessive lethal mutations, but no visible mutations. One of the lethal mutations, d29A6, was identified as an overlapping deficiency extending from 66F3 to 67B1. An additional 6000 diepoxybutane-treated chromosomes were screened for lethality over d29A6, yielding another four lethal mutations within the 67A2-B1 subinterval. These 78 lethal mutations, along with two others isolated in other laboratories, define 23 essential loci--6 within the 67A2-B1 subinterval and 17 within the 67A2 to D11-13 subinterval. Many of these loci appear to be required for imaginal development only, exhibiting late larval to pharate adult lethal phases. Examination of the 67A2-B1 lethal complementation groups for (1) earlier onset of lethality following a heat shock, (2) missing or altered small hsps on two-dimensional protein gels, and (3) restoration of viability by transformed wild-type copies of the small hsp genes indicates that none of these mutations affect the small hsps. On the basis of this analysis and the known homology of the genes, we conclude that the small hsps are functionally equivalent.

This article has been cited by other articles:

				W. Wang, C. Cronmiller, and D. L. Brautigan Maternal Phosphatase Inhibitor-2 Is Required for Proper Chromosome Segregation and Mitotic Synchrony During Drosophila Embryogenesis Genetics, August 1, 2008; 179(4): 1823 - 1833. [Abstract] [Full Text] [PDF]

				M. Matsuno, H. Kose, M. Okabe, and Y. Hiromi TFIIH controls developmentally-regulated cell cycle progression as a holocomplex. Genes Cells, November 1, 2007; 12(11): 1289 - 1300. [Abstract] [Full Text] [PDF]

				J. Kirchner, S. Gross, D. Bennett, and L. Alphey The Nonmuscle Myosin Phosphatase PP1{beta} (flapwing) Negatively Regulates Jun N-Terminal Kinase in Wing Imaginal Discs of Drosophila Genetics, April 1, 2007; 175(4): 1741 - 1749. [Abstract] [Full Text] [PDF]

				X. Bi, D. Srikanta, L. Fanti, S. Pimpinelli, R. Badugu, R. Kellum, and Y. S. Rong Drosophila ATM and ATR checkpoint kinases control partially redundant pathways for telomere maintenance PNAS, October 18, 2005; 102(42): 15167 - 15172. [Abstract] [Full Text] [PDF]

				G. HERNANDEZ, P. VAZQUEZ-PIANZOLA, J. M. SIERRA, and R. RIVERA-POMAR Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos RNA, November 18, 2004; 10(11): 1783 - 1797. [Abstract] [Full Text] [PDF]

				R. Gandhi, S. Bonaccorsi, D. Wentworth, S. Doxsey, M. Gatti, and A. Pereira The Drosophila Kinesin-like Protein KLP67A Is Essential for Mitotic and Male Meiotic Spindle Assembly Mol. Biol. Cell, January 1, 2004; 15(1): 121 - 131. [Abstract] [Full Text] [PDF]

				P. E. D. Lachance, M. Miron, B. Raught, N. Sonenberg, and P. Lasko Phosphorylation of Eukaryotic Translation Initiation Factor 4E Is Critical for Growth Mol. Cell. Biol., March 15, 2002; 22(6): 1656 - 1663. [Abstract] [Full Text] [PDF]

				J. H. Mansfield, J. E. Wilhelm, and T. Hazelrigg Ypsilon Schachtel, a Drosophila Y-box protein, acts antagonistically to Orb in the oskar mRNA localization and translation pathway Development, January 1, 2002; 129(1): 197 - 209. [Abstract] [Full Text] [PDF]

				C. Verrijzer, K Yokomori, J. Chen, and R Tjian Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA Science, May 13, 1994; 264(5161): 933 - 941. [Abstract] [PDF]

				K. Matthews, D Rees, and T. Kaufman A functionally specialized alpha-tubulin is required for oocyte meiosis and cleavage mitoses in Drosophila Development, January 3, 1993; 117(3): 977 - 991. [Abstract] [PDF]