- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Gudenus, R.
- Articles by Thuriaux, P.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Gudenus, R.
- Articles by Thuriaux, P.
Genetics, Vol 119, 517-526, Copyright © 1988
INVESTIGATIONS |
Conditional Mutants of RPC160, the Gene Encoding the Largest Subunit of RNA Polymerase C in Saccharomyces cerevisiae
R. Gudenus, S. Mariotte, A. Moenne, A. Ruet, S. Memet, J. M. Buhler, A. Sentenac and P. Thuriaux
Present address: Immuno AG, Uferstrasse 15, A2304, Orth an der Donau, Austria.
A 18.4-kb fragment of the yeast genome containing the gene of the largest subunit of RNA polymerase C (RPC160) was cloned by hybridization to a previously isolated fragment of that gene. RPC160 maps on chromosome XV, tightly linked but not allelic to the essential gene TSM8740. Temperature sensitive (ts) mutant alleles were constructed by in vitro mutagenesis with NaHSO(3) and substituted for the wild-type allele on the chromosome. Four of them were unambiguously identified as rpc160 mutants by failure to complement a fully defective mutation rpc160::URA3. The faithful transcription of a yeast tRNA gene by mutant cell-free extracts is strongly reduced as compared to wild-type. In vivo, the rpc160 mutations specifically affect the synthesis of tRNA in a temperature sensitive way, with comparatively little effect on the synthesis of 5S rRNA and no effect on 5.8S rRNA. An unlinked mutation (pci1-3) suppresses the temperature sensitive phenotype of the rpc160-41 mutation.
This article has been cited by other articles:
 |
|
 |
|
  K. Pluta, O. Lefebvre, N. C. Martin, W. J. Smagowicz, D. R. Stanford, S. R. Ellis, A. K. Hopper, A. Sentenac, and M. Boguta Maf1p, a Negative Effector of RNA Polymerase III in Saccharomyces cerevisiae Mol. Cell. Biol., August 1, 2001; 21(15): 5031 - 5040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-F. Briand, F. Navarro, O. Gadal, and P. Thuriaux Cross Talk between tRNA and rRNA Synthesis in Saccharomyces cerevisiae Mol. Cell. Biol., January 1, 2001; 21(1): 189 - 195. [Abstract] [Full Text]
|
|
|
|
|
|
M.-L. Ferri, G. Peyroche, M. Siaut, O. Lefebvre, C. Carles, C. Conesa, and A. Sentenac A Novel Subunit of Yeast RNA Polymerase III Interacts with the TFIIB-Related Domain of TFIIIB70 Mol. Cell. Biol., January 15, 2000; 20(2): 488 - 495. [Abstract] [Full Text]
|
|
|
|
|
|
E. Deprez, R. Arrebola, C. Conesa, and A. Sentenac A Subunit of Yeast TFIIIC Participates in the Recruitment of TATA-Binding Protein Mol. Cell. Biol., December 1, 1999; 19(12): 8042 - 8051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. CHEDIN, M.L. FERRI, G. PEYROCHE, J.C. ANDRAU, S. JOURDAIN, O. LEFEBVRE, M. WERNER, C. CARLES, and A. SENTENAC The Yeast RNA Polymerase III Transcription Machinery: A Paradigm for Eukaryotic Gene Activation Cold Spring Harb Symp Quant Biol, January 1, 1998; 63(0): 381 - 390. [Abstract] [PDF]
|
|