- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Montelone, B. A.
- Articles by Malone, R. E.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Montelone, B. A.
- Articles by Malone, R. E.
Genetics, Vol 119, 289-301, Copyright © 1988
INVESTIGATIONS |
Spontaneous Mitotic Recombination in Yeast: The Hyper-Recombinational rem1 Mutations Are Alleles of the RAD3 Gene
B. A. Montelone, M. F. Hoekstra and R. E. Malone
Department of Biology, University of Iowa, Iowa City, Iowa 52242
The RAD3 gene of Saccharomyces cerevisiae is required for UV excision-repair and is essential for cell viability. We have identified the rem1 mutations (enhanced spontaneous mitotic recombination and mutation) of Saccharomyces cerevisiae as alleles of RAD3 by genetic mapping, complementation with the cloned wild-type gene, and DNA hybridization. The high levels of spontaneous mitotic gene conversion, crossing over, and mutation conferred upon cells by the rem1 mutations are distinct from the effects of all other alleles of RAD3. We present preliminary data on the localization of the rem1 mutations within the RAD3 gene. The interaction of the rem1 mutant alleles with a number of radiation-sensitive mutations is also different than the interactions reported for previously described (UV-sensitive) alleles of RAD3. Double mutants of rem1 and a defect in the recombination-repair pathway are inviable, while double mutants containing UV-sensitive alleles of RAD3 are viable. The data presented here demonstrate that: (1) rem1 strains containing additional mutations in other excision-repair genes do not exhibit elevated gene conversion; (2) triple mutants containing rem1 and mutations in both excision-repair and recombination-repair are viable; (3) such triple mutants containing rad52 have reduced levels of gene conversion but wild-type frequencies of crossing over. We have interpreted these observations in a model to explain the effects of rem1. Consistent with the predictions of the model, we find that the size of DNA from rem1 strains, as measured by neutral sucrose gradients, is smaller than wild type.
This article has been cited by other articles:
 |
|
 |
|
  M. S. Navarro, L. Bi, and A. M. Bailis A Mutant Allele of the Transcription Factor IIH Helicase Gene, RAD3, Promotes Loss of Heterozygosity in Response to a DNA Replication Defect in Saccharomyces cerevisiae Genetics, July 1, 2007; 176(3): 1391 - 1402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Lephart and P. T. Magee Effect of the Major Repeat Sequence on Mitotic Recombination in Candida albicans Genetics, December 1, 2006; 174(4): 1737 - 1744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Morey, P. W. Doetsch, and S. Jinks-Robertson Delineating the Requirements for Spontaneous DNA Damage Resistance Pathways in Genome Maintenance and Viability in Saccharomyces cerevisiae Genetics, June 1, 2003; 164(2): 443 - 455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Enloe, A. Diamond, and A. P. Mitchell A Single-Transformation Gene Function Test in Diploid Candida albicans J. Bacteriol., October 15, 2000; 182(20): 5730 - 5736. [Abstract] [Full Text]
|
|
|
|
 |
|
 B.-S. Lee, L. Bi, D. J. Garfinkel, and A. M. Bailis Nucleotide Excision Repair/TFIIH Helicases Rad3 and Ssl2 Inhibit Short-Sequence Recombination and Ty1 Retrotransposition by Similar Mechanisms Mol. Cell. Biol., April 1, 2000; 20(7): 2436 - 2445. [Abstract] [Full Text]
|
|
|
|
|
|
M. Fasullo, J. Koudelik, P. AhChing, P. Giallanza, and C. Cera Radiosensitive and Mitotic Recombination Phenotypes of the Saccharomyces cerevisiae dun1 Mutant Defective in DNA Damage-Inducible Gene Expression Genetics, July 1, 1999; 152(3): 909 - 919. [Abstract] [Full Text]
|
|
|
|
|
|
S. Maines, M. C. Negritto, X. Wu, G. M. Manthey, and A. M. Bailis Novel Mutations in the RAD3 and SSL1 Genes Perturb Genome Stability by Stimulating Recombination Between Short Repeats in Saccharomyces cerevisiae Genetics, November 1, 1998; 150(3): 963 - 976. [Abstract] [Full Text]
|
|
|
|
|
|
B.-S. Lee, C. P. Lichtenstein, B. Faiola, L. A. Rinckel, W. Wysock, M. J. Curcio, and D. J. Garfinkel Posttranslational Inhibition of Ty1 Retrotransposition by Nucleotide Excision Repair/Transcription Factor TFIIH Subunits Ssl2p and Rad3p Genetics, April 1, 1998; 148(4): 1743 - 1761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fasullo, T. Bennett, P. Ahching, and J. Koudelik The Saccharomyces cerevisiae RAD9 Checkpoint Reduces the DNA Damage-Associated Stimulation of Directed Translocations Mol. Cell. Biol., March 1, 1998; 18(3): 1190 - 1200. [Abstract] [Full Text]
|
|
|
|
|
|
B. J. Merrill and C. Holm The RAD52 Recombinational Repair Pathway is Essential in pol30 (PCNA) Mutants That Accumulate Small Single-Stranded DNA Fragments During DNA Synthesis Genetics, February 1, 1998; 148(2): 611 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hoekstra, R. Liskay, A. Ou, A. DeMaggio, D. Burbee, and F Heffron HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA Science, August 30, 1991; 253(5023): 1031 - 1034. [Abstract] [PDF]
|
|