P Element Insertions and Rearrangements at the singed Locus of Drosophila melanogaster

INVESTIGATIONS

P Element Insertions and Rearrangements at the singed Locus of Drosophila melanogaster

H. Roiha, G. M. Rubin and K. O'Hare
Department of Biochemistry, University of California, Berkeley, California 94720 Present address: Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143.

DNA from the singed gene of Drosophila melanogaster was isolated using an inversion between a previously cloned P element at cytological location 17C and the hypermutable allele singed-weak. Five out of nine singed mutants examined have alterations in their DNA maps in this region. The singed locus is a hotspot for mutation during P-M hybrid dysgenesis, and we have analyzed 22 mutations induced by P-M hybrid dysgenesis. All 22 have a P element inserted within a 700-bp region. The precise positions of 10 P element insertions were determined and they define 4 sites within a 100-bp interval. During P-M hybrid dysgenesis, the singed-weak allele is destabilized, producing two classes of phenotypically altered derivatives at high frequency. In singed-weak, two defective P elements are present in a ``head-to-head'' or inverse tandem arrangement. Excision of one element results in a more extreme singed bristle phenotype while excision of the other leads to a wild-type bristle phenotype.

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				H. Schenkel, S. Hanke, C. De Lorenzo, R. Schmitt, and B. M. Mechler P Elements Inserted in the Vicinity of or Within the Drosophila snRNP SmD3 Gene Nested in the First Intron of the Ornithine Decarboxylase Antizyme Gene Affect Only the Expression of SmD3 Genetics, June 1, 2002; 161(2): 763 - 772. [Abstract] [Full Text] [PDF]

				M. J. Simmons, K. J. Haley, C. D. Grimes, J. D. Raymond, and J. B. Niemi A hobo Transgene That Encodes the P-Element Transposase in Drosophila melanogaster: Autoregulation and Cytotype Control of Transposase Activity Genetics, May 1, 2002; 161(1): 195 - 204. [Abstract] [Full Text] [PDF]

				B. Timakov, X. Liu, I. Turgut, and P. Zhang Timing and Targeting of P-Element Local Transposition in the Male Germline Cells of Drosophila melanogaster Genetics, March 1, 2002; 160(3): 1011 - 1022. [Abstract] [Full Text] [PDF]

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