Genetics, Vol 118, 461-470, Copyright © 1988

INVESTIGATIONS

A Stable Genomic Source of P Element Transposase in Drosophila melanogaster

H. M. Robertson, C. R. Preston, R. W. Phillis, D. M. Johnson-Schlitz, W. K. Benz and W. R. Engels
Present address: Department of Entomology, University of Illinois, Urbana, Illinois 61801.

A single P element insert in Drosophila melanogaster, called P[ry(+){Delta}2-3](99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for ``helper'' plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.

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